Song Changxiao, Li Jingxian, Wang Futing, Peng Ziyang, Shen Yan, Huang Chongyang, Deng Suping, Yang Hongfen, Yang Dan, Cai Ren, Tan Weihong
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha, Hunan 410082, China.
Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang, Hunan 421001, China.
ACS Nano. 2025 Jul 22;19(28):26161-26169. doi: 10.1021/acsnano.5c07691. Epub 2025 Jul 9.
A multifunctional electrochemiluminescence (ECL) coreaction accelerator, AuAgPt nanoframes (NFs), is described for use in an ECL aptasensor for highly sensitive aflatoxin B1 (AFB1) detection. As a signal quencher, the broad UV-vis absorption spectrum of AuAgPt nanosheets (NSs) overlaps the ECL emission spectrum of g-CN@Au, triggering an ECL resonance energy transfer (ECL-RET). By the adjustment of the dosage of hydrogen peroxide (HO), the AuAgPt NSs are transformed into AuAgPt NFs because HO etches Ag in AuAgPt NSs into Ag, which disrupts the RET process. The as-formed AuAgPt NFs act as a coreaction accelerator to enhance the ECL response of the g-CN@Au/KSO system. Without AFB1, the Ag-dependent DNAzyme is inactive, and a strong ECL signal is observed. After AFB1 is added, the AFB1 aptamer targets AFB1 and the DNAzyme active site is exposed. As-generated Ag further activates DNAzyme to cut the substrate strand (S-DNA), which causes AuAgPt NFs to detach from the electrode surface and the ECL signal to significantly decrease. Under optimal conditions, the proposed ECL aptasensor exhibits high sensitivity with a limit of detection (LOD) of 0.11 fg/mL in the range of 1 fg/mL to 1 μg/mL for AFB1 detection.
一种多功能电化学发光（ECL）共反应促进剂——金银铂纳米框架（NFs），被用于一种ECL适体传感器中，用于高灵敏度检测黄曲霉毒素B1（AFB1）。作为信号猝灭剂，金银铂纳米片（NSs）的宽紫外-可见吸收光谱与g-CN@Au的ECL发射光谱重叠，引发ECL共振能量转移（ECL-RET）。通过调整过氧化氢（HO）的用量，金银铂NSs转变为金银铂NFs，因为HO将金银铂NSs中的Ag蚀刻成Ag，这破坏了RET过程。形成的金银铂NFs作为共反应促进剂增强了g-CN@Au/KSO体系的ECL响应。在没有AFB1的情况下，依赖于Ag的DNAzyme无活性，观察到强ECL信号。加入AFB1后，AFB1适体靶向AFB1，DNAzyme活性位点暴露。生成的Ag进一步激活DNAzyme切割底物链（S-DNA），这导致金银铂NFs从电极表面脱离，ECL信号显著降低
