Patel Shubham, Chaturvedi Aman, Dubey Nazneen, Shrivastava Abhishek, Ganeshpurkar Aditya
Shri Ram Institute of Technology-Pharmacy, Jabalpur, India.
ILIVER. 2022 Mar 10;1(1):72-77. doi: 10.1016/j.iliver.2022.03.001. eCollection 2022 Mar.
Phyto-constituents are widely recognized for hepatoprotective effects. Ascorbic acid is extensively studied for the radical scavenging effect. The objective of the present study was to evaluate the hepatoprotective effect of ascorbic acid against isoniazid and rifampicin-induced liver damage in rats.
Wistar rats were used in the present study. Hepato-cellular damage was produced by the administration of isoniazid (100 mg/kg) and rifampicin (100 mg/kg) for 21 days. Ascorbic acid was administered in the dose of 50, 100, and 200 mg/kg body weight. At the end of the study, blood was collected and biochemical studies were performed to determine antioxidant status.
Ascorbic acid administration (50, 100, and 200 mg/kg body weight) caused restoration of serum glutamic oxaloacetic transaminase, serum glutamic-pyruvic transaminase, and serum alkaline phosphatase. The level of superoxide dismutase and catalase were also restored due to the administration of ascorbic acid. There was a decrement in the expression of tumor necrosis factor-α, interleukin-1β, interleukin-6, malondialdehyde and nitric oxide.
The outcome of the study established ascorbic acid as a notable hepatoprotective effect. The radical scavenging and cytokine suppression effect is the possible mechanism associated with the hepatoprotective effect of ascorbic acid.
植物成分因其肝脏保护作用而被广泛认可。抗坏血酸因具有自由基清除作用而得到广泛研究。本研究的目的是评估抗坏血酸对异烟肼和利福平诱导的大鼠肝损伤的肝脏保护作用。
本研究使用了Wistar大鼠。通过给予异烟肼（100mg/kg）和利福平（100mg/kg）21天来造成肝细胞损伤。以50、100和200mg/kg体重的剂量给予抗坏血酸。在研究结束时，采集血液并进行生化研究以确定抗氧化状态。
给予抗坏血酸（50、100和200mg/kg体重）可使血清谷草转氨酶、血清谷丙转氨酶和血清碱性磷酸酶恢复正常。由于给予抗坏血酸，超氧化物歧化酶和过氧化氢酶的水平也得以恢复。肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6、丙二醛和一氧化氮的表达有所下降。
本研究结果证实抗坏血酸具有显著的肝脏保护作用。自由基清除和细胞因子抑制作用可能是与抗坏血酸肝脏保护作用相关的机制。
