Kim Gayeong, Park Beomseok, Ji Seunghyeon, Ferdiansyah Mokhammad Khoiron, Kwon Yonghwi, Cha Myeongseong, Gaddapara Manasa, Oh Hwagyun, Kim Kwangpyo
Department of Food Science and Technology, College of Agriculture and Life Sciences, Jeonbuk National University, 54896, Jeonju, Jeollabuk-do, Korea.
Department of Agricultural Convergence Technology, College of Agriculture and Life Sciences, Jeonbuk National University, 54896, Jeonju, Jeollabuk-do, Korea.
Arch Virol. 2025 Jul 10;170(8):176. doi: 10.1007/s00705-025-06357-8.
Pasteurella multocida, especially capsular serotype A, is a zoonotic pathogen that is found mainly in livestock. While antibiotics are commonly used to treat bacterial infections, bacteriophage therapy has become an important alternative due to the issue of antibiotic resistance. Although four virulent P. multocida-infecting phages have been described, their interactions with their hosts have not been extensively studied. In this study, a novel virulent phage, PMP1, was isolated from a livestock waste treatment facility in Korea. This phage has a short, non-contractile tail with an isometric head and specifically lyses toxA-negative serotype A strains but can adsorb to toxA-positive strains. PMP1 has a genome of 38,049 bp in length with no genes related to lysogeny. Bioinformatic analysis and transmission electron microscopy suggest that a toxA-containing prophage could be involved in the restriction-modification system; however, the correlationof the presence of toxA with phage resistance was not clearly established. The structural proteins were identified for the first time in a P. multocida-infecting phage by LC-MS/MS analysis, and phylogenetic analysis revealed that PMP1 could be a member of new species in the genus Wuhanvirus. These findings suggest that this phage should be investigated further as a potential anti-P. multocida agent.
多杀性巴氏杆菌，尤其是A群荚膜血清型，是一种主要存在于家畜中的人畜共患病原体。虽然抗生素常用于治疗细菌感染，但由于抗生素耐药性问题，噬菌体疗法已成为一种重要的替代方法。尽管已经描述了四种感染多杀性巴氏杆菌的烈性噬菌体，但它们与宿主的相互作用尚未得到广泛研究。在本研究中，从韩国一家家畜粪便处理设施中分离出一种新型烈性噬菌体PMP1。这种噬菌体有一条短的、不可收缩的尾巴和一个等轴状头部，能特异性裂解toxA阴性的A群血清型菌株，但可吸附于toxA阳性菌株。PMP1基因组长度为38,049 bp，没有与溶原性相关的基因。生物信息学分析和透射电子显微镜表明，一个含toxA的前噬菌体可能参与了限制修饰系统；然而，toxA的存在与噬菌体抗性之间的相关性尚未明确确立。通过LC-MS/MS分析首次在一种感染多杀性巴氏杆菌的噬菌体中鉴定出结构蛋白，系统发育分析表明PMP1可能是武汉病毒属新物种的成员。这些发现表明，该噬菌体作为一种潜在的抗多杀性巴氏杆菌制剂应进一步研究。
