虎螈和墨西哥钝口螈染色体之间的带型差异。

Banding differences between tiger salamander and axolotl chromosomes.

作者信息

Cuny R, Malacinski G M

出版信息

Can J Genet Cytol. 1985 Oct;27(5):510-4. doi: 10.1139/g85-076.

Abstract

The Hoechst 33258 - Giemsa banding patterns were compared on axolotl (Ambystoma mexicanum Shaw) and axolotl - tiger salamander (Ambystoma tigrinum Green) species hybrid prophase chromosomes. Approximately 369 bands per haploid chromosome set were seen in the axolotl and about 344 bands in the tiger salamander. In the haploid set of 14 chromosomes, chromosome 3 has a constant short or q-arm terminal constriction at the location of the nucleolar organizer. Chromosomes 14 Z and W carry the sex determinants, the female being the heterogametic sex (ZW). The banding patterns of chromosomes 1, 6, 11, and 14 Z of the two species are apparently indistinguishable by our banding method. In the axolotl, chromosome 9 has a small long or p-arm terminal deletion. In the tiger salamander, the remaining 10 chromosomes have terminal or internal deletions. No translocations or inversions seem to have occurred since the gene pool separation of the two closely related species.

摘要

对美西螈（墨西哥钝口螈，肖氏）和美西螈 - 虎螈（虎纹钝口螈，格林）物种杂交前期染色体上的Hoechst 33258 - 吉姆萨带型进行了比较。在美西螈中，每个单倍体染色体组大约可见369条带，在虎螈中约为344条带。在14条染色体的单倍体组中，3号染色体在核仁组织区位置有恒定的短臂或q臂末端缢痕。14号Z染色体和W染色体携带性别决定因子，雌性为异配性别（ZW）。通过我们的带型分析方法，这两个物种的1号、6号、11号和14号Z染色体的带型显然无法区分。在美西螈中，9号染色体有一个小的长臂或p臂末端缺失。在虎螈中，其余10条染色体有末端或内部缺失。自这两个近缘物种的基因库分离以来，似乎未发生易位或倒位。