Zymovets Valeriia, Rakhimova Olena, Schmidt Alexej, Bronnec Vicky, Limanska Nataliia, Brundin Malin, Kelk Peyman, Landström Maréne, Vestman Nelly Romani
Department of Odontology, Umeå University, Umeå, Sweden.
Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.
iScience. 2025 Apr 11;28(5):112412. doi: 10.1016/j.isci.2025.112412. eCollection 2025 May 16.
Oral health in immature permanent teeth with traumatic injuries is particularly vulnerable, and regenerative endodontic treatment (RET) using stem cells from the apical papilla (SCAP) holds potential for root development and tissue regeneration. However, bacterial persistence, especially , poses a challenge to successful treatment outcomes. To address this, we evaluated the probiotic for its co-aggregative and anti-adhesive properties against . An aggregation test demonstrated effective co-aggregation between the probiotic and opportunistic strains. Additionally, flow cytometry analysis revealed that binding to SCAP was significantly reduced when the concentration was nine times higher. To substantiate these findings, an gut model was used, where immunofluorescence imaging and culture-based methods confirmed decreased adhesion at both 1:1 and 9:1 probiotic-to-opportunistic ratios. These results highlight B16 as a promising probiotic strain to improve RET outcomes.
外伤后未成熟恒牙的口腔健康特别脆弱，使用根尖乳头干细胞（SCAP）进行的再生牙髓治疗（RET）对牙根发育和组织再生具有潜力。然而，细菌的持续存在，尤其是[具体细菌未提及]，对治疗成功结果构成挑战。为解决这一问题，我们评估了益生菌[具体益生菌未提及]对[具体细菌未提及]的共聚集和抗粘附特性。一项[具体细菌未提及]聚集试验证明了益生菌与机会性菌株之间有效的共聚集。此外，流式细胞术分析显示，当[具体细菌未提及]浓度高九倍时，其与SCAP的结合显著减少。为证实这些发现，使用了[具体细菌未提及]肠道模型，其中免疫荧光成像和基于培养的方法证实，在益生菌与机会性菌株比例为1:1和9:1时，[具体细菌未提及]的粘附均减少。这些结果突出了[具体益生菌未提及]B16作为一种有前景的益生菌菌株，可改善RET结果。
