多菌株益生菌上清液根管内用药对牙模型粪肠球菌生物膜的抗菌效果。
Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model.
机构信息
Division of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
Division of Operative Dentistry, Conservative Dentistry Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
出版信息
BMC Oral Health. 2023 Apr 20;23(1):228. doi: 10.1186/s12903-023-02914-2.
BACKGROUND
To assess the antibacterial activity of multi-strain probiotics supernatants (MSP); Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus acidophilus as an intracanal medication on Enterococcus faecalis (E. faecalis) biofilm in a tooth model.
METHODS
Sixty extracted human single-rooted teeth with single canals were instrumented, sterilized, and inoculated with E. faecalis. After 21 days of incubation, four specimens were randomly selected to validate the biofilm formation by scanning electron microscope (SEM). The remaining specimens were randomly divided (n = 14), according to the intracanal medication (ICM) received into: Ca(OH): calcium hydroxide paste (35% Ultra Cal XS Ca(OH)), Probiotics supernatants: MSP in poloxamer gel vehicle Poloxamer: poloxamer gel vehicle and, Control: E. faecalis biofilm only. The tested groups were further subdivided into two equal subgroups (n = 7) according to the incubation period (24 h and 7 days). Shaved dentin chips were obtained and collected by H-files and paper points, respectively for bacterial culture. The antibacterial activity was assessed after each incubation period quantitatively and qualitatively using bacterial colony-forming units per milliliter (CFUs/ml) and SEM, respectively.
RESULTS
The lowest CFUs/ml was found in Ca (OH) with a significant difference compared to other groups after 24 h. After 7 days, a similar outcome was found with a further significant reduction of CFUs/ml in all groups with no statistical difference between Ca(OH) and probiotics supernatants groups. Ca (OH) and Probiotics supernatants groups showed a significant (p < 0.05) percentage of overall bacterial reduction (100.00 ± 0.00% and 70.30 ± 12.95%, respectively) compared to poloxamer and control groups (27.80 ± 14.45 and 28.29 ± 19.79). SEM images showed a bacteria-free state in the Ca(OH) group after 7 days while few bacteria were found in the probiotics supernatants group. An extensive invasion of bacteria was found in poloxamer and controls groups.
CONCLUSION
MSP has a potential antibacterial effect on E. faecalis growth closely similar to the routinely used Ca (OH).
背景
评估多菌株益生菌上清液（MSP）；植物乳杆菌、鼠李糖乳杆菌和嗜酸乳杆菌作为根管内药物对牙模型中粪肠球菌（E. faecalis）生物膜的抗菌活性。
方法
将 60 颗经过单根单根管器械处理、消毒并接种粪肠球菌的人离体单根牙进行孵育 21 天后，随机选择 4 个样本通过扫描电子显微镜（SEM）验证生物膜的形成。其余样本根据接受的根管内药物（ICM）随机分为 14 个组（n=14）：氢氧化钙：氢氧化钙糊剂（35% Ultra Cal XS Ca(OH)）、益生菌上清液：多聚体凝胶载体中的 MSP、对照：仅粪肠球菌生物膜。测试组根据孵育时间（24 小时和 7 天）进一步细分为两个相等的亚组（n=7）。用 H 锉和纸尖分别获取和收集削切牙本质屑，用于细菌培养。分别使用每毫升菌落形成单位（CFU/ml）和 SEM 对每个孵育期的抗菌活性进行定量和定性评估。
结果
在 24 小时后，发现 CFU/ml 最低的是氢氧化钙组，与其他组相比有显著差异。7 天后，所有组的 CFU/ml 均进一步显著降低，但氢氧化钙组和益生菌上清液组之间无统计学差异。氢氧化钙组和益生菌上清液组的总细菌减少率有显著差异（p<0.05）（分别为 100.00±0.00%和 70.30±12.95%），而多聚体凝胶载体组和对照组分别为 27.80±14.45%和 28.29±19.79%。7 天后，SEM 图像显示氢氧化钙组细菌呈无菌状态，而益生菌上清液组仅发现少量细菌。多聚体凝胶载体组和对照组中发现了广泛的细菌入侵。
结论
MSP 对粪肠球菌生长具有潜在的抗菌作用，与常规使用的氢氧化钙非常相似。
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