Gao Ning, Sun Jialin, Zhao Wei, Duan Lihui, Cai Hongyao, Liu Bo, Cheng Yupeng
Key Laboratory of Basic and Application Research of Beiyao, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, Heilongjiang 150040, P.R. China.
School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.
Oncol Lett. 2025 Jul 2;30(3):419. doi: 10.3892/ol.2025.15165. eCollection 2025 Sep.
Neuroblastoma (NB) is the most common pediatric malignant neoplasm. Saikosaponin A (SSa), a compound with potential therapeutic effects against this disease, inhibits the proliferation, metastasis and invasion of NB cells. However, its molecular mechanism remains elusive. The present study examined changes in gene expression in SK-N-AS NB cells after SSa administration using RNA sequencing. Subsequently, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Search Tool for the Retrieval of Interacting Genes/Proteins were used to analyze the differentially expressed genes between the treatment and control groups. Quantitative PCR technology confirmed the expression of the identified critical genes. The results identified multiple significant biological processes, including 717 GO terms and 55 KEGG pathways, constructing a 96-gene protein-protein interaction network, with as the most relevant player in anti-NB mechanisms. The activities of SSa against NB were closely related to the regulations of the following genes: and Additionally, the PI3K-Akt signaling pathway was downregulated in the KEGG enriched results. Therefore, the results of the present study improves the critical understanding of the anti-NB mechanism of SSa and lays a foundation for its clinical application against NB.
神经母细胞瘤（NB）是最常见的儿科恶性肿瘤。柴胡皂苷A（SSa）是一种对该疾病具有潜在治疗作用的化合物，可抑制NB细胞的增殖、转移和侵袭。然而，其分子机制仍不清楚。本研究使用RNA测序检测了SSa给药后SK-N-AS NB细胞中基因表达的变化。随后，利用基因本体论（GO）、京都基因与基因组百科全书（KEGG）和检索相互作用基因/蛋白质的搜索工具来分析治疗组和对照组之间的差异表达基因。定量PCR技术证实了所鉴定关键基因的表达。结果确定了多个重要的生物学过程，包括717个GO术语和55条KEGG通路，构建了一个96个基因的蛋白质-蛋白质相互作用网络，其中 是抗NB机制中最相关的参与者。SSa对NB的作用与以下基因的调控密切相关： 和 此外，KEGG富集结果中PI3K-Akt信号通路被下调。因此，本研究结果提高了对SSa抗NB机制的关键认识，并为其抗NB的临床应用奠定了基础。
