尿毒症血清和正常血清中的荧光物质。

Fluorescent substances in uremic and normal serum.

作者信息

Shaykh M, Bazilinski N, McCaul D S, Ahmed S, Dubin A, Musiala T, Dunea G

出版信息

Clin Chem. 1985 Dec;31(12):1988-92.

Abstract

We measured the fluorescence, at various excitation (Ex) and emission (Em) wavelengths, of serum ultrafiltrates and fractions of serum resolved by chromatography on Sephadex G15, studying both normal subjects and patients in chronic renal failure requiring hemodialysis. We found hitherto undescribed fluorescence at Ex 380 nm/Em 440 nm and Ex 400 nm/Em 460 nm, the intensity being greatly increased in patients with chronic renal failure in comparison with normal subjects (p less than 0.005). This fluorescence persisted unaltered when serum was filtered through membranes having cutoffs ranging from 10 000 to 500 Da. Each serum fraction resolved by gel chromatography demonstrated a characteristic fluorescence, which was generally much more intense in uremics. The most intense fluorescence (Ex 380 nm/Em 440 nm and Ex 400 nm/Em 460 nm) was emitted in the higher-Mr fractions.

摘要

我们测量了血清超滤液以及经葡聚糖凝胶G15柱色谱分离的血清各组分在不同激发（Ex）和发射（Em）波长下的荧光，研究对象包括正常受试者以及需要血液透析的慢性肾衰竭患者。我们发现了此前未描述过的在Ex 380 nm/Em 440 nm和Ex 400 nm/Em 460 nm处的荧光，与正常受试者相比，慢性肾衰竭患者的这种荧光强度显著增加（p < 0.005）。当血清通过截留分子量范围为10000至500 Da的膜进行过滤时，这种荧光保持不变。凝胶色谱分离得到的每个血清组分都显示出特征性荧光，尿毒症患者的荧光通常要强得多。最强的荧光（Ex 380 nm/Em 440 nm和Ex 400 nm/Em 460 nm）出现在较高分子量组分中。