Liu Yule, Jian Jingjing, Xu Lingxin, Meng Linyi, Yang Fan, Li Shihao, Yan Junxin
College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China.
J Agric Food Chem. 2025 Jul 23;73(29):18186-18197. doi: 10.1021/acs.jafc.5c03530. Epub 2025 Jul 11.
Arbuscular mycorrhizal fungi (AMF) influence plant-insect interactions, yet how they modulate volatile organic compounds (VOCs) to affect insect behavior remains unclear. This study integrates physiological assays, GC-MS volatile metabolomics, and insect bioassays to investigate how (Fm) and (Ri) inoculation affects growth and its resistance to . The results showed that Fm and Ri inoculation both promoted plant growth but differed in defense strategies. Ri inoculation increased the tannin content (16.98%) and polyphenol oxidase activity (40.52%), whereas Fm inoculation showed neutral effects. VOC profiling revealed that Ri inoculation upregulated leaf monoterpenes and phenylpropanoids, while Fm inoculation reduced sesquiterpenes (e.g., β-selinene). Insect bioassays showed that Ri inoculation inhibited the insect growth, whereas Fm inoculation promoted the insect growth and enhanced its feeding preference. These results revealed that AMF triggers a plant growth-defense trade-off, with sesquiterpenoid regulation critical for insect behavioral shifts. The findings provide a basis for sustainable pest management using AMF-plant symbiosis.
丛枝菌根真菌（AMF）会影响植物与昆虫之间的相互作用，然而它们如何调节挥发性有机化合物（VOCs）以影响昆虫行为仍不清楚。本研究结合生理测定、气相色谱 - 质谱挥发性代谢组学和昆虫生物测定，以探究接种摩西斗管囊霉（Fm）和根内球囊霉（Ri）如何影响植物生长及其对[具体害虫名称缺失]的抗性。结果表明，接种Fm和Ri均促进了植物生长，但防御策略有所不同。接种Ri增加了单宁含量（16.98%）和多酚氧化酶活性（40.52%），而接种Fm则显示出中性效应。挥发性有机化合物分析表明，接种Ri上调了叶片单萜和苯丙烷类化合物，而接种Fm则降低了倍半萜（如β-芹子烯）。昆虫生物测定表明，接种Ri抑制了昆虫生长，而接种Fm促进了昆虫生长并增强了其取食偏好。这些结果表明，AMF引发了植物生长 - 防御权衡，倍半萜类化合物的调节对昆虫行为转变至关重要。这些发现为利用AMF - 植物共生进行可持续害虫管理提供了依据。
