Shi Feng, Wang Xinghao, He Xue, Xu Tianle, Jiang Mingguo, Chang Wei, Song Fuqiang
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, China.
School of Marine Sciences and Biotechnology, Guangxi Key Laboratory of Polysaccharide Materials and Modifications, Guangxi Minzu University, Nanning, China.
Appl Environ Microbiol. 2025 Jul 23;91(7):e0055825. doi: 10.1128/aem.00558-25. Epub 2025 Jun 24.
Arbuscular mycorrhizal fungi (AMF) are a key group of fungi closely associated with agricultural production within soil microbial communities. However, large-scale propagation of AMF inoculum faces various challenges, limiting our ability to obtain and utilize these inocula on a broad scale. To address this, we designed a monolayer mesh cultivation system employing a hydroponic approach for propagating arbuscular mycorrhizal fungi, specifically . We conducted a comparative analysis of quality and inoculation efficiency between the water culture inoculum (w-Ri) and traditional soil-based inoculum (s-Ri). Our findings revealed the following. (i) The propagation cycle of w-Ri inoculum is 35 days and only 23% of the 150-day cycle required for s-Ri inoculum. (ii) The spore density, viability, and purity of w-Ri inoculum are 5.25 times, 1.09 times, and 1.26 times higher, respectively, than those of s-Ri inoculum. (iii) The w-Ri inoculants demonstrate effects on enhancing rice biomass, root morphology, and photosynthesis that are consistent with those of the s-Ri inoculants, while requiring only 10% of the application rate of the s-Ri inoculants. These results provide crucial theoretical references for establishing a pure and efficient arbuscular mycorrhizal fungus propagation system and its promotion and application.IMPORTANCEThe development of a monolayer mesh hydroponic cultivation system for propagating offers a significant advancement in overcoming the challenges of large-scale AMF inoculum production, which is critical for enhancing agricultural sustainability. The comparative analysis of water culture-based (w-Ri) and traditional soil-based (s-Ri) inoculum demonstrates the superior efficiency of the w-Ri system in terms of propagation speed, spore density, and inoculum quality, highlighting its potential for large-scale application in farming practices. The findings that w-Ri inoculants are equally effective in promoting plant growth while requiring only a fraction of the application rate of s-Ri inoculants underscore the potential for reducing both cost and environmental impact in agricultural inoculation practices.
丛枝菌根真菌（AMF）是土壤微生物群落中与农业生产密切相关的一类关键真菌。然而，丛枝菌根真菌接种剂的大规模繁殖面临各种挑战，限制了我们在广泛范围内获取和利用这些接种剂的能力。为解决这一问题，我们设计了一种采用水培方法的单层网室栽培系统来繁殖丛枝菌根真菌。我们对水培接种剂（w-Ri）和传统土壤接种剂（s-Ri）的质量和接种效率进行了比较分析。我们的研究结果如下：（i）w-Ri接种剂的繁殖周期为35天，仅为s-Ri接种剂150天周期的23%。（ii）w-Ri接种剂的孢子密度、活力和纯度分别比s-Ri接种剂高5.25倍、1.09倍和1.26倍。（iii）w-Ri接种剂在促进水稻生物量、根系形态和光合作用方面的效果与s-Ri接种剂一致，但用量仅为s-Ri接种剂的10%。这些结果为建立纯净高效的丛枝菌根真菌繁殖系统及其推广应用提供了关键的理论参考。重要性开发一种用于繁殖的单层网室水培栽培系统，在克服大规模丛枝菌根真菌接种剂生产的挑战方面取得了重大进展，这对提高农业可持续性至关重要。对水培（w-Ri）和传统土壤（s-Ri）接种剂的比较分析表明，w-Ri系统在繁殖速度、孢子密度和接种剂质量方面具有更高的效率，突出了其在农业实践中大规模应用的潜力。w-Ri接种剂在促进植物生长方面同样有效，而用量仅为s-Ri接种剂的一小部分，这一发现强调了在农业接种实践中降低成本和环境影响的潜力。
