Bian Xia, Wen Xiaoye, Huang Yongfei, Fan Zhefeng
School of Chemistry and Material Science, Shanxi Normal University, Taiyuan, 030031, People's Republic of China.
School of Public Health, Shanxi Medical University, Taiyuan, 030001, China.
Mikrochim Acta. 2025 Jul 11;192(8):494. doi: 10.1007/s00604-025-07374-9.
A facile one-step hydrothermal synthesis of dual-emission fluorescent carbon dots (D-CDs) is presented using giemsa stain and malic acid as precursors. The resulting D-CDs exhibit unique long-wavelength dual-emission properties with exceptional pH sensitivity across a broad range (3.0-10.0), showing particularly strong linear correlation (R = 0.9927) in the physiologically relevant pH window (5.0-9.0). This pH-dependent behavior, combined with their excellent photostability, makes them ideal probes for biological imaging applications. The D-CDs also demonstrate selective detection capability for vitamin B (VB) through inner filter effect-mediated quenching of the green emission channel (520 nm), achieving a wide linear detection range (2-120 μM) with a low detection limit (0.96 μM). Comprehensive biocompatibility assessments confirmed low cytotoxicity (> 86% cell viability at 60 μg·mL⁻) and successful application in both cellular systems and zebrafish models. Their unique combination of dual-emission properties, pH sensitivity, selective molecular recognition, and excellent biosafety establishes D-CDs as a versatile platform for physiological monitoring and biomedical imaging applications.
本文报道了一种以吉姆萨染色剂和苹果酸为前驱体，通过简便的一步水热法合成双发射荧光碳点（D-CDs）。所得的D-CDs展现出独特的长波长双发射特性，在较宽的pH范围（3.0 - 10.0）内具有出色的pH敏感性，在生理相关的pH窗口（5.0 - 9.0）中表现出特别强的线性相关性（R = 0.9927）。这种pH依赖性行为，再加上其出色的光稳定性，使其成为生物成像应用的理想探针。D-CDs还通过内滤光效应介导的绿色发射通道（520 nm）猝灭，展示了对维生素B（VB）的选择性检测能力，实现了宽线性检测范围（2 - 120 μM）和低检测限（0.96 μM）。全面的生物相容性评估证实其具有低细胞毒性（在60 μg·mL⁻时细胞活力> 86%），并成功应用于细胞系统和斑马鱼模型。它们独特的双发射特性、pH敏感性、选择性分子识别和出色的生物安全性相结合，使D-CDs成为生理监测和生物医学成像应用的通用平台。
