Jung Hye-Ri, Lee Young Ju
College of Veterinary Medicine & Institute for Veterinary Biomedical Science, Kyungpook National University, Daegu 41566, Korea.
Avian Disease Division, Animal and Plant Quarantine Agency, Gimcheon-si 39660, Gyeongsangbuk-do, Korea.
Avian Dis. 2025 Jun;69(2):144-151. doi: 10.1637/aviandiseases-D-24-00038.
Nontyphoidal spp. potentially lead to economic loss, thereby threatening the poultry industry; moreover, they are major pathogens that can cause human illness. In this study, were isolated from the internal organs of chickens that had died within one week of age in 132 commercial farms of five integrated broiler operations. were isolated from 35 (26.5%) farms, and the most frequently detected serovar was serovar Enteritidis, detected in 19 (14.4%) farms, followed by serovars Typhimurium (4 farms, 3.0%), . Senftenberg (3 farms, 2.3%), . Agona (2 farms, 1.5%), . Montevideo (2 farms, 1.5%), . Infantis (2 farms, 1.5%), . Thompson (2 farms, 1.5%), and . Bareilly (1 farm, 0.8%) ( < 0.05). In particular, . Enteritidis was identified on farms (4.5%-22.2%) of all five operations. The prevalence of resistance to nalidixic acid (97.7%-100%) was significantly higher than that of resistance to other antimicrobials (0.0%-44.4%) in all five operations ( < 0.05), and the prevalence of multidrug resistance showed significant differences among the five integrated operations ( < 0.05). . Enteritidis was divided into 18 pulsed-field gel electrophoresis pattern types; however, confirming the epidemiological relationship proved challenging. Moreover, 9 (14.5%) among 62 . Enteritidis isolates harbored 2 kinds of β-lactamase genes, (2 isolates) and (7 isolates), and 4 (6.5%) . Enteritidis isolates harbored 2 kinds of integrase genes: class 1 integron (2 isolates) and class 2 integron (2 isolates). Most of the 20 virulence genes tested were detected in more than 93.5% of . Enteritidis isolates, but the and genes were only detected in 2 isolates (3.2%) and 1 isolate (1.6%), respectively. This study indicates that various spp., including . Enteritidis and . Typhimurium, are persistent in commercial broiler farms via vertical or horizontal transmission.
非伤寒沙门氏菌可能导致经济损失，从而威胁家禽业；此外，它们是可导致人类疾病的主要病原体。在本研究中，从五个一体化肉鸡养殖场的132个商业农场中一周龄内死亡的鸡的内脏中分离出沙门氏菌。在35个（26.5%）农场中分离出沙门氏菌，最常检测到的血清型是肠炎沙门氏菌血清型，在19个（14.4%）农场中检测到，其次是鼠伤寒沙门氏菌血清型（4个农场，3.0%）、森夫滕贝格沙门氏菌血清型（3个农场，2.3%）、阿哥纳沙门氏菌血清型（2个农场，1.5%）、蒙得维的亚沙门氏菌血清型（2个农场，1.5%）、婴儿沙门氏菌血清型（2个农场，1.5%）、汤普森沙门氏菌血清型（2个农场，1.5%）和巴雷利沙门氏菌血清型（1个农场，0.8%）（P<0.05）。特别是，在所有五个养殖场的农场中均鉴定出肠炎沙门氏菌血清型（4.5%-22.2%）。在所有五个养殖场中，对萘啶酸的耐药率（97.7%-100%）显著高于对其他抗菌药物的耐药率（0.0%-44.4%）（P<0.05），并且多重耐药率在五个一体化养殖场之间存在显著差异（P<0.05）。肠炎沙门氏菌血清型分为18种脉冲场凝胶电泳图谱类型；然而，证实其流行病学关系具有挑战性。此外，62株肠炎沙门氏菌分离株中有9株（14.5%）携带2种β-内酰胺酶基因，即blaTEM（2株）和blaSHV（7株），4株（6.5%）肠炎沙门氏菌分离株携带2种整合酶基因：1类整合子（2株）和2类整合子（2株）。所检测的20个毒力基因中的大多数在93.5%以上的肠炎沙门氏菌分离株中被检测到，但spvC基因和spvR基因仅分别在2株（3.2%）和1株（1.6%）中被检测到。本研究表明，包括肠炎沙门氏菌血清型和鼠伤寒沙门氏菌血清型在内的各种沙门氏菌通过垂直或水平传播在家禽商业养殖场中持续存在。
