Yang Wenhao, Xing Zheng, Wang Xin, Xu Zhen, Jiang Xiaolian, Xia Jiang, Qiu Lin, Xu Jianda, Wang Jianhao
School of Pharmacy & School of Medical and Health Engineering, Changzhou University, Changzhou, Jiangsu 213164, PR China.
School of Pharmacy & School of Medical and Health Engineering, Changzhou University, Changzhou, Jiangsu 213164, PR China; Department of Pharmacy, The Second People's Hospital of Changzhou, The Third Affliated Hospital of Nanjing Medical University, Changzhou Medical Center, Nanjing Medical University, Changzhou, Jiangsu 213004, PR China.
Int J Biol Macromol. 2025 Jul 9:145840. doi: 10.1016/j.ijbiomac.2025.145840.
Wound healing in diabetic patients is often impaired due to a combination of tissue inflammation, excessive reactive oxygen species (ROS), insufficient neovascularization, and pH imbalances. To overcome these challenges, we designed a multifunctional hydrogel system capable of responding to the wound microenvironment and enabling the controlled release of plant-derived extracellular vesicles (PDEVs) to accelerate diabetic wound healing. The hydrogel was constructed using recombinant human Type III collagen (rhCOL3) grafted with 3-aminophenylboronic acid (APBA) to gain the microenvironment response ability. Additionally, PDEVs derived from Houttuynia cordata thunb (HC-EVs) were embedded within the hydrogel to improve the biological ability. This HC-EVs-loaded BA-rhCOL3 hydrogel (HC-EVs@B-CH) demonstrates excellent tissue adhesion, self-healing properties, and notable antioxidant and anti-inflammatory effects. By regulating the wound microenvironment, it significantly enhances diabetic wound healing through improved angiogenesis, increased collagen deposition, and reduced inflammation. Mechanism studies have shown that HC-EVs@B-CH can regulating both NF-κB and YAP pathways, which may be the underlying biological mechanism for its role in promoting the healing of diabetic wounds. In summary, this HC-EVs@B-CH offers a multifunctional, innovative solution for diabetic wound care. Its ability to address key barriers in wound healing highlights its potential as a next-generation wound dressing for effective diabetic wound management.
由于组织炎症、过量活性氧（ROS）、新生血管形成不足和pH失衡等多种因素共同作用，糖尿病患者的伤口愈合常常受到损害。为了克服这些挑战，我们设计了一种多功能水凝胶系统，该系统能够对伤口微环境做出响应，并实现植物源细胞外囊泡（PDEVs）的控释，以加速糖尿病伤口愈合。该水凝胶由接枝了3-氨基苯硼酸（APBA）的重组人III型胶原蛋白（rhCOL3）构建而成，以获得微环境响应能力。此外，将源自鱼腥草（HC-EVs）的PDEVs包埋在水凝胶中，以提高其生物学性能。这种负载HC-EVs的BA-rhCOL3水凝胶（HC-EVs@B-CH）表现出优异的组织粘附性、自愈性能以及显著的抗氧化和抗炎作用。通过调节伤口微环境，它通过改善血管生成、增加胶原蛋白沉积和减轻炎症，显著促进糖尿病伤口愈合。机制研究表明，HC-EVs@B-CH可以调节NF-κB和YAP途径，这可能是其促进糖尿病伤口愈合作用的潜在生物学机制。总之，这种HC-EVs@B-CH为糖尿病伤口护理提供了一种多功能的创新解决方案。其解决伤口愈合关键障碍的能力突出了其作为下一代有效管理糖尿病伤口的伤口敷料的潜力。
