Anagnostides A A, Chadwick V S, Selden A C, Barr J, Maton P N
Clin Sci (Lond). 1985 Sep;69(3):259-63. doi: 10.1042/cs0690259.
To determine the functional significance of physiological plasma concentrations of cholecystokinin, five volunteers each received graded doses of intravenous infusions of cholecystokinin octapeptide (CCK-8). At each dose plasma concentrations of CCK-8 were determined and pancreatic and biliary outputs were measured. Threshold plasma concentrations of CCK-8 for augmenting pancreatic trypsin secretion were undetectable (less than 3 pmol/l), and maximal trypsin output of 21.9 +/- 1.95 k-i.u./30 min was produced by 17.1 +/- 6.4 pmol of CCK-8/1. Calculated halfmaximal output was produced by 4.7 pmol of CCK-8/1. Maximal trypsin output during infusions of CCK-8 was significantly less than that after a combination of the CCK-like peptide, caerulein, and secretin (32.95 +/- 2.16 k-i.u./30 min, P less than 0.001). Biliary bile acid and bilirubin outputs were significantly augmented only when plasma concentrations of CCK-8 were greater than 5 pmol/l. Plasma concentrations of CCK-8 in the low picomolar range exert significant effects on pancreatic and biliary secretion. CCK-8 fulfills the criteria for a circulating hormone.
为了确定生理血浆浓度的胆囊收缩素的功能意义，五名志愿者每人接受了不同剂量的胆囊收缩素八肽（CCK-8）静脉输注。在每个剂量下，测定CCK-8的血浆浓度，并测量胰腺和胆汁的分泌量。增加胰腺胰蛋白酶分泌的CCK-8阈值血浆浓度无法检测到（低于3 pmol/l），17.1±6.4 pmol的CCK-8/1产生了21.9±1.95 k-i.u./30分钟的最大胰蛋白酶分泌量。计算得出，4.7 pmol的CCK-8/1产生了半数最大分泌量。输注CCK-8期间的最大胰蛋白酶分泌量显著低于CCK样肽、蛙皮素和促胰液素联合使用后的分泌量（32.95±2.16 k-i.u./30分钟，P<0.001）。仅当CCK-8的血浆浓度大于5 pmol/l时，胆汁酸和胆红素的胆汁分泌量才会显著增加。低皮摩尔范围内的CCK-8血浆浓度对胰腺和胆汁分泌有显著影响。CCK-8符合循环激素的标准。
