Boero R, Quarello F, Guarena C, Rosati C, Piccoli G
Clin Sci (Lond). 1985 Dec;69(6):709-12. doi: 10.1042/cs0690709.
The effects of a 2 litre intravenous infusion of saline (0.9% NaCl solution) over 3 h on erythrocyte transmembrane sodium transport were studied in 12 normal human subjects. After saline infusion a significant (P less than 0.01) reduction of both outward Na+,K+ pump- and Na+,K+ cotransport-mediated Na+ effluxes was observed. The Na+,Li+ countertransport rate and the passive Na+ permeability did not change. The incubation of the subjects' erythrocytes, obtained on a separate occasion, with their own plasma taken after the saline infusion, induced an inhibition of both Na+,K+ pump and Na+,K+ cotransport outward sodium fluxes. The percentage decrease after incubation was closely correlated with the percentage reduction induced by the saline infusion in vivo (r = 0.93 for the pump and r = 0.96 for cotransport; P less than 0.01). These data suggest that extracellular fluid volume expansion affects the release of circulating factors modulating sodium transport by the Na+,K+ pump and by Na+,K+ cotransport.
在12名正常人体受试者中研究了3小时内静脉输注2升生理盐水（0.9%氯化钠溶液）对红细胞跨膜钠转运的影响。输注生理盐水后，观察到由外向Na⁺,K⁺泵和Na⁺,K⁺协同转运介导的Na⁺外流均显著（P<0.01）减少。Na⁺,Li⁺逆向转运速率和被动Na⁺通透性未发生变化。在另一个时间点获取受试者的红细胞，并用输注生理盐水后采集的自身血浆进行孵育，可诱导Na⁺,K⁺泵和Na⁺,K⁺协同转运的外向钠通量均受到抑制。孵育后的减少百分比与体内输注生理盐水诱导的减少百分比密切相关（泵的r = 0.93，协同转运的r = 0.96；P<0.01）。这些数据表明细胞外液容量扩张会影响调节Na⁺,K⁺泵和Na⁺,K⁺协同转运的钠转运的循环因子的释放。
