Atallah Elie, Mahayri Tiziana Maria, Mrázek Jakub, Bovera Fulvia, Piccolo Giovanni, Fliegerová Kateřina Olša, Moniello Giuseppe
Department of Veterinary Medicine, University of Sassari, Via Vienna, 2, Sassari, 07100, Italy.
Department of Veterinary Medicine and Animal Sciences, University of Milan, Via dell'Università, 6, Lodi, 26900, Italy.
Sci Rep. 2025 Jul 11;15(1):25081. doi: 10.1038/s41598-025-09936-6.
In the present study, we examined the influence of the dietary inclusion of black soldier fly (BSF) larvae meal on the diversity and composition of the bacterial community in the caecum of Barbary partridges (Alectoris barbara). A total of 54 partridges were divided equally into three treatment groups. The control group (C) received a diet containing corn-soybean meals and the two experimental groups received diets in which soybean meal protein was partially substituted with BSF larvae meal at proportions of 25% (H25) and 50% (H50). The bacterial community of the caecal samples was analysed in 30 slaughtered animals (10 per group) at 64 days of age. High-throughput sequencing targeting the V4-V5 region of the 16 S rRNA gene was used. Firmicutes were the most abundant phylum in all studied categories. This phylum was dominated by the families Ruminococcaceae and Lachnospiraceae. The caecal microbiota was significantly altered at the genus level. The linear discriminant analysis effect size (LefSe) analysis for the differential taxa abundance revealed several significant dissimilarities between the control group (C) and the groups with 25% and 50% insect meal replacement, with 13 and 20 taxa with significantly different abundances, respectively. Several of these taxa are associated with gut health, fiber fermentation, and metabolic functions, indicating a biological importance of the observed microbial shifts. Compared with the control group, the partridges fed 25% BSF larvae meal had a significantly higher bacterial phylogenetic abundance and richness, which may contribute to improved gut health and a more stable microbial environment. The beta diversity measures revealed that all three groups of animals were significantly spatially separated. The results demonstrated the significant impact of black soldier fly larvae meal on the caecal microbiota of Barbary partridges. The positive influence of the insect meal used was indicated by increased bacterial diversity in the H25 group and increased relative abundance of several potentially beneficial genera in both experimental groups.
在本研究中，我们检测了日粮中添加黑水虻（BSF）幼虫粉对石鸡（Alectoris barbara）盲肠细菌群落多样性和组成的影响。总共54只石鸡被平均分为三个处理组。对照组（C）饲喂含玉米-豆粕的日粮，两个实验组饲喂用BSF幼虫粉分别按25%（H25）和50%（H50）的比例部分替代豆粕蛋白的日粮。在64日龄时，对30只屠宰动物（每组10只）的盲肠样本细菌群落进行了分析。采用针对16S rRNA基因V4-V5区域的高通量测序。厚壁菌门是所有研究类别中最丰富的菌门。该菌门以瘤胃球菌科和毛螺菌科为主。盲肠微生物群在属水平上发生了显著变化。对差异分类群丰度的线性判别分析效应大小（LefSe）分析显示，对照组（C）与25%和50%昆虫粉替代组之间存在若干显著差异，分别有13个和20个分类群丰度显著不同。其中一些分类群与肠道健康、纤维发酵和代谢功能有关，表明所观察到的微生物变化具有生物学重要性。与对照组相比，饲喂25% BSF幼虫粉的石鸡细菌系统发育丰度和丰富度显著更高，这可能有助于改善肠道健康和形成更稳定的微生物环境。β多样性测量结果表明，三组动物在空间上显著分离。结果表明，黑水虻幼虫粉对石鸡盲肠微生物群有显著影响。所用昆虫粉的积极影响表现为H25组细菌多样性增加，两个实验组中几个潜在有益属的相对丰度增加。
