Graves S S, Evans D L, Dawe D L
Comp Immunol Microbiol Infect Dis. 1985;8(1):43-51. doi: 10.1016/0147-9571(85)90053-0.
Channel catfish demonstrate a shift in the tissue distribution of nonspecific cytotoxic cells (NCC) when infected with the protozoan parasite, Ichthyophthirius multifiliis. NCC, isolated from head kidney (HK) tissues (hemopoietic organ) or peripheral blood leukocytes, were assessed for cytotoxic activity against NC-37 (a transformed mammalian cell line). NCC activity from HK tissue of moribund I. multifiliis-infected fish was depressed compared to HK-NCC activity in uninfected or I. multifillis-immune fish. The activity of NCC, isolated from the peripheral blood of moribund I. multifiliis-infected fish was significantly greater than the NCC activity in peripheral blood from either immune or uninfected fish. Chromium-51 release assays were combined with effector and target conjugate assays to determine killing capacity (Vmax) and affinity (Km) for target cells of peripheral blood NCC from moribund I. multifiliis-infected and uninfected fish. These experiments indicated that the peripheral blood from the moribund infected fish contained an increased percentage of active NCC with increased killing capacity and target cell affinity compared to peripheral blood NCC activity of uninfected fish.
当感染原生动物寄生虫多子小瓜虫时，斑点叉尾鮰的非特异性细胞毒性细胞（NCC）的组织分布会发生变化。从头部肾脏（HK）组织（造血器官）或外周血白细胞中分离出的NCC，被评估对NC-37（一种转化的哺乳动物细胞系）的细胞毒性活性。与未感染或多子小瓜虫免疫鱼的HK-NCC活性相比，濒死的多子小瓜虫感染鱼的HK组织中的NCC活性降低。从濒死的多子小瓜虫感染鱼的外周血中分离出的NCC活性明显高于免疫或未感染鱼外周血中的NCC活性。将铬-51释放试验与效应细胞和靶细胞结合试验相结合，以确定濒死的多子小瓜虫感染鱼和未感染鱼外周血NCC对靶细胞的杀伤能力（Vmax）和亲和力（Km）。这些实验表明，与未感染鱼的外周血NCC活性相比，濒死感染鱼的外周血中活性NCC的百分比增加，杀伤能力和靶细胞亲和力增强。
