Wang Yu-Xuan, Jia Tao, Wang Zi-Rui, Wang Jin-Long, Zhang Ze-Dong, Wu Ze-Xuan, Gao Wen-Wei, Zhu Xing-Quan, Liu Qing
Laboratory of Parasitic Diseases, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
The Yunnan Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Yunnan Agricultural University, Kunming 650201, China.
Animals (Basel). 2025 Jun 27;15(13):1899. doi: 10.3390/ani15131899.
Several trichomonad species have already been reported from cattle, including and . However, there is currently a lack of information concerning the prevalence of trichomonad species in cattle in Shanxi Province, North China. In this study, 761 fecal samples from cattle across three counties in Shanxi Province, namely Qi, Jishan, and Shanyin, were examined for the presence of and DNA through a nested PCR assay targeting a specific segment of the small subunit ribosomal RNA (SSU rRNA) gene. The results showed that the total prevalence of in cattle was found to be 74.5%, with region and sex identified as risk factors for infection. exhibited an overall prevalence of 3.0%, with strong associations observed between infection and both region and age. Sequencing analysis indicated that some isolates and all isolates were identical to those reported previously based on the analysis of SSU rRNA sequences, while certain isolates exhibited minor allelic variations. These results enhance our understanding of the geographical distribution and genetic characterization of and in cattle.
已有报道称牛身上存在几种毛滴虫，包括[具体名称1]和[具体名称2]。然而，目前关于中国北方山西省牛群中毛滴虫种类流行情况的信息匮乏。在本研究中，通过针对小亚基核糖体RNA（SSU rRNA）基因特定片段的巢式PCR检测法，对山西省祁县、稷山和山阴三个县的761份牛粪便样本进行检测，以确定是否存在[具体名称1]和[具体名称2]的DNA。结果显示，牛群中[具体名称1]的总体感染率为74.5%，地区和性别被确定为感染的风险因素。[具体名称2]的总体感染率为3.0%，感染与地区和年龄之间均存在显著关联。测序分析表明，部分[具体名称1]分离株和所有[具体名称2]分离株与先前基于SSU rRNA序列分析所报道的相同，而某些[具体名称1]分离株表现出微小的等位基因变异。这些结果加深了我们对牛群中[具体名称1]和[具体名称2]的地理分布及遗传特征的理解。
