Tian Caihong, Zhang Junyi, Li Guoping, Huang Jianrong, Wu Shaoying, Yin Xinming, Feng Hongqiang
Henan Key Laboratory of Agricultural Pest Monitoring and Control, Key Laboratory of Integrated Crop Pests Management on Crops in Southern Region of North China, Ministry of Agriculture and Rural Affairs, International Joint Research Laboratory for Crop Protection of Henan, Biopesticide Engineering Research Center of Henan Province, Agricultural Microbiology Innovation Center of Henan Province, No. 0 Entomological Radar Field Scientific Observation and Research Station of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Sanya Nanfan Research Institute, Hainan University, Sanya 572025, China.
Plants (Basel). 2025 Jun 25;14(13):1944. doi: 10.3390/plants14131944.
Among various plants, corn is the primary host damaged by J. E. Smith (Lepidoptera: Noctuidae). After long-term regional colonization, its larvae feed on sweet waxy corn and fresh corn for extended periods. A question arises: Does long-term feeding on different corn varieties affect their rhythms? Currently, there are no reports addressing these issues. To facilitate the formulation of effective prevention and control measures, Zhengdan 958 and Zhenghuangnuo were selected as representative varieties of normal and sweet waxy corn, respectively, for laboratory experiments. were fed the leaves of these two corn types over nine consecutive generations, thereby establishing distinct strains associated with each corn variety. Additionally, a strain fed an artificial diet served as the control group. Through a comparative analysis of the emergence, movement, nutritional foraging, dormancy, mating, and oviposition behaviors of adult fall armyworms from different populations, differences in the six behavioral peak times among the strains were identified. RT-qPCR analysis indicated significant differences in the expression levels of four circadian clock genes across different populations and tissues of the fall armyworm. Feeding on different host plants influenced the expression of circadian clock genes and their associated behavioral rhythms. Our study showed that sweet corn is more conducive to pupation, mating, and oviposition. Because of these differences in adult insect rhythms, sweet corn may have an impact on the reproduction of fall armyworms in the Huang-Huai-Hai corn-planting region.
在各种植物中，玉米是受草地贪夜蛾（鳞翅目：夜蛾科）危害的主要寄主。经过长期的区域定殖后，其幼虫长时间取食甜糯玉米和鲜食玉米。由此产生一个问题：长期取食不同玉米品种会影响它们的节律吗？目前，尚无针对这些问题的报道。为便于制定有效的防控措施，分别选取郑单958和正黄糯作为普通玉米和甜糯玉米的代表品种进行实验室试验。让其连续9代取食这两种玉米的叶片，从而建立了与每个玉米品种相关的不同品系。此外，用人工饲料喂养的品系作为对照组。通过对不同种群的成虫草地贪夜蛾的羽化、活动、营养觅食、休眠、交配和产卵行为进行比较分析，确定了各品系间6种行为高峰期的差异。RT-qPCR分析表明，草地贪夜蛾不同种群和组织中4个生物钟基因的表达水平存在显著差异。取食不同寄主植物会影响生物钟基因的表达及其相关的行为节律。我们的研究表明，甜玉米更有利于化蛹、交配和产卵。由于成虫昆虫节律存在这些差异，甜玉米可能会对黄淮海玉米种植区草地贪夜蛾的繁殖产生影响。
