Vitko Sandra, Leljak-Levanić Dunja, Bauer Nataša, Vidaković-Cifrek Željka
Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia.
Plants (Basel). 2025 Jun 27;14(13):1969. doi: 10.3390/plants14131969.
Plant responses to heat stress include complex transcriptional networks and protein regulations in which BTB/POZ-MATH (BPM) proteins participate as a part of ubiquitin-mediated protein degradation. contains six genes involved in responses to environmental changes, including heat. Seedlings overexpressing (), seedlings with downregulation of , , , and () and wild type were exposed to 37 °C for 6 h. Treatment caused stronger decline of photosynthesis in than in and wild type, although all seedlings recovered after 24 h at 24 °C. The activity of the antioxidant enzymes catalase, guaiacol peroxidase, and ascorbate peroxidase remained unchanged in , but increased in and wild type. Heat stress induced HSP70 and HSP90 in all seedlings but expression remained notably higher in after recovery. and expression increased in all seedlings immediately after stress, with the strongest induction in . In and wild type, expression was induced immediately after exposure, while , , , and were upregulated in wild type after recovery. In seedlings, expression decreased and expression increased immediately after treatment at 37 °C for 6 h. The results suggest that BPM proteins modulate heat stress response by influencing photosynthesis, activation of antioxidant enzymes, accumulation of HSPs, and expression of heat-responsive genes, thus contributing to the different physiological strategies observed in . lines with altered expression of genes.
植物对热胁迫的响应包括复杂的转录网络和蛋白质调控，其中BTB/POZ-MATH（BPM）蛋白作为泛素介导的蛋白质降解的一部分参与其中。包含六个参与环境变化响应（包括热响应）的基因。过表达（）的幼苗、下调、、、和（）的幼苗以及野生型幼苗在37°C下暴露6小时。处理导致中光合作用的下降比和野生型更强，尽管所有幼苗在24°C下放置24小时后都恢复了。抗氧化酶过氧化氢酶、愈创木酚过氧化物酶和抗坏血酸过氧化物酶的活性在中保持不变，但在和野生型中增加。热胁迫在所有幼苗中诱导了HSP70和HSP90，但恢复后中其表达仍显著更高。胁迫后所有幼苗中立即增加，其中诱导最强。在和野生型中，暴露后立即诱导表达，而恢复后野生型中、、、和上调。在幼苗中，37°C处理6小时后，表达下降而表达增加。结果表明，BPM蛋白通过影响光合作用、抗氧化酶的激活、HSPs的积累和热响应基因的表达来调节热胁迫响应，从而导致在基因表达改变的品系中观察到不同的生理策略。
