Ji Hyo Seong, Bang Seoung Gun, Ahn Min-A, Kim Gayeon, Kim Eunhui, Eom Seung Hee, Hyun Tae Kyung
Department of Industrial Plant Science and Technology, College of Agricultural, Life and Environmental Sciences, Chungbuk National University, Cheongju 28644, Korea.
Antioxidants (Basel). 2021 May 20;10(5):815. doi: 10.3390/antiox10050815.
Superoxide dismutases (SODs) are key antioxidant enzymes that can detoxify the superoxide radicals generated by various stresses. Although various plant SODs have been suggested to improve stress tolerance, SODs in garlic, an economically important vegetable grown worldwide, remain relatively unknown. In this study, we found that heat stress strongly induced the activities of Cu/ZnSODs, FeSODs, and MnSODs in garlic leaves. In addition, we cloned four garlic () and suggest that heat stress-increased SOD activity was reflected at least by the induction of these . The results of the agro-infiltration assay suggested that the cloned encoded functional SOD enzymes belonging to the Cu/ZnSOD and MnSOD families. As a first step toward understanding the enzymatic antioxidant system in garlic plants, our results provide a solid foundation for an in-depth analysis of the physiological functions of the AsSOD family.
超氧化物歧化酶（SOD）是关键的抗氧化酶，可清除各种胁迫产生的超氧自由基。尽管已有研究表明多种植物SOD可提高胁迫耐受性，但大蒜（一种在全球广泛种植的重要经济蔬菜）中的SOD仍相对鲜为人知。在本研究中，我们发现热胁迫强烈诱导了大蒜叶片中铜/锌超氧化物歧化酶（Cu/ZnSOD）、铁超氧化物歧化酶（FeSOD）和锰超氧化物歧化酶（MnSOD）的活性。此外，我们克隆了四个大蒜超氧化物歧化酶基因，并表明热胁迫诱导的SOD活性至少部分是由这些基因的诱导所反映。农杆菌浸润试验结果表明，克隆的基因编码属于Cu/ZnSOD和MnSOD家族的功能性SOD酶。作为了解大蒜植物酶促抗氧化系统的第一步，我们的结果为深入分析大蒜超氧化物歧化酶（AsSOD）家族的生理功能提供了坚实的基础。
