Xu Lin, Deng Zhaolei, Hu Shan, Liu Qian, Luan Qifu, Yang Chunxia
Jiangxi Academy of Forestry, Nanchang 330013, China.
Jiangxi Provincial Key Laboratory of Improved Variety Breeding and Efficient Utilization of Native Tree Species, Nanchang 330013, China.
Plants (Basel). 2025 Jun 29;14(13):1985. doi: 10.3390/plants14131985.
, a key economic conifer in southern China, requires efficient propagation methods to meet demand for elite germplasm in resin and timber production. While somatic embryogenesis-based plant regeneration has been successfully achieved in , large-scale production remains challenging. Our results demonstrate that the genotype of significantly influences the induction rate of embryogenic callus. During somatic embryo maturation, the liquid-solid induction method increased the number of mature embryos by 25.85 times. Maturation efficiency was further enhanced by a 3-week pretreatment followed by the application of 9 mg/L ABA, 0.5 mg/L PSK, and 6 mg/L COS. Additionally, the incorporation of activated carbon significantly promoted both the maturation and germination of somatic embryos. In the somatic embryo maturation stage, 1 g/L activated carbon induced 288.67 mature embryos per gram of embryogenic callus, resulting in a total of 1452 embryos. During germination, the application of 4 g/L activated carbon achieved a germination rate of 63%, and the survival rate of somatic embryo-derived seedlings reached 85%. This study not only identifies the optimal conditions for somatic embryogenesis in but also establishes an efficient protocol for somatic embryo maturation induction, providing a crucial scientific foundation for the rapid propagation and seedling production of .
[物种名称]是中国南方一种关键的经济针叶树，需要高效的繁殖方法来满足树脂和木材生产中对优良种质的需求。虽然基于体细胞胚胎发生的植株再生已在[物种名称]中成功实现，但大规模生产仍具有挑战性。我们的结果表明，[物种名称]的基因型显著影响胚性愈伤组织的诱导率。在体细胞胚胎成熟过程中，液固诱导法使成熟胚数量增加了25.85倍。通过3周预处理，然后施用9 mg/L脱落酸、0.5 mg/L植物硫肽激素和6 mg/L壳寡糖，成熟效率进一步提高。此外，添加活性炭显著促进了体细胞胚胎的成熟和萌发。在体细胞胚胎成熟阶段，1 g/L活性炭每克胚性愈伤组织诱导出288.67个成熟胚，共获得1452个胚。在萌发过程中，施用4 g/L活性炭发芽率达到63%，体细胞胚胎来源的幼苗成活率达到85%。本研究不仅确定了[物种名称]体细胞胚胎发生的最佳条件，还建立了一套高效的体细胞胚胎成熟诱导方案，为[物种名称]的快速繁殖和苗木生产提供了关键的科学依据。
