Guan Chunjing, Gao Yike, Wang Ziyi, Zhang Qixiang
Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, School of Landscape Architecture, Beijing Forestry University, No. 35 Qinghua East Road, Haidian District, Beijing 100083, China.
Plants (Basel). 2025 Jun 30;14(13):1996. doi: 10.3390/plants14131996.
spp. exhibit distinct flower opening times, categorized into nocturnal and diurnal types. Previous studies have demonstrated that the circadian clock and () genes play crucial roles in regulating flowering in . However, the key genes that integrate flowering pathways remain largely unknown. To address this gap, we identified potential homologs of the () gene in . A yeast one-hybrid assay revealed that HfCOL2 and HfLHY directly bind to the and promoters, thereby activating transcription. The expression analysis reveals that expression rhythms not only display opposing patterns between nocturnal and diurnal opening types of but also between leaf and flower tissues. The peak expression of in flowers aligns closely with the respective opening times of diurnally and nocturnally flowering . The overexpression of in tobacco plants led to early flowering and prolonged flower longevity. In , the gene plays a pivotal role not only in photoperiod-induced flowering but also in the circadian rhythm-mediated regulation of flower opening time. Due to the limited availability of plant materials exhibiting distinct flower opening rhythms, research in this area has been constrained. Identifying the key genes in the flowering pathway of can facilitate a better understanding of the mechanisms by which plants respond to circadian rhythms.
某些物种表现出不同的花朵开放时间，可分为夜间型和白天型。先前的研究表明，生物钟和（）基因在调节（某植物）开花过程中起着关键作用。然而，整合开花途径的关键基因在很大程度上仍然未知。为了填补这一空白，我们在（某植物）中鉴定了（）基因的潜在同源物。酵母单杂交试验表明，HfCOL2和HfLHY直接与（某基因）和（另一基因）的启动子结合，从而激活（某基因）转录。表达分析表明，（某基因）的表达节律不仅在（某植物）夜间和白天开放类型之间呈现相反模式，而且在叶和花组织之间也呈现相反模式。（某基因）在花中的表达峰值与白天和夜间开花（某植物）各自的开放时间密切相关。在烟草植株中过表达（某基因）导致早花并延长了花朵寿命。在（某植物）中，（某基因）不仅在光周期诱导的开花中起关键作用，而且在生物钟介导的花朵开放时间调节中也起关键作用。由于表现出不同花朵开放节律的植物材料有限，该领域的研究受到了限制。鉴定（某植物）开花途径中的关键基因有助于更好地理解植物对生物钟节律作出反应的机制。
