Zhang Hao, Liu Xiaodong, Li Cunyan, Fang Linzhi, Gai Chaoyue, Zeng Rensen, Wang Qiongli, Song Yuanyuan, Chen Daoqian
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Key Laboratory of Ministry of Agriculture and Rural Affairs of Biological Breeding for Fujian and Taiwan Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Fujian Provincial University Key Laboratory of Crop Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel). 2025 Jul 6;14(13):2066. doi: 10.3390/plants14132066.
Silicon (Si) protects plants against insect herbivores; however, the underlying mechanisms remain unclear. Polyamines (PAs) play a crucial role in plant-insect interactions. Here, the involvement of Si in putrescine (Put) metabolism and its role in rice resistance against striped stem borer (SSB, Walker) were investigated. The results showed that SSB larval infestation led to a substantial accumulation of free Put in rice seedlings. Si application increased rice resistance against SSB and repressed the SSB attack-induced accumulation of Put, in parallel with a decreased expression of Put biosynthesis genes encoding arginine decarboxylase ( and ). Moreover, Si application had no significant effect on the wounding-induced expression of and , but attenuated the further elevation in the transcription of and induced by SSB larvae oral secretion. Simultaneously, Si addition reduced the Put and spermidine contents in SSB-attacked plants. Furthermore, the exogenous application of Put attenuated Si-enhanced resistance against SSB larvae, whereas exogenous D-arginine, an inhibitor of ADC, showed similar effects to Si on rice resistance against SSB. Our findings indicate that Si improves rice resistance to SSB, at least partly by reducing herbivory-stimulated putrescine accumulation.
硅（Si）可保护植物免受昆虫食草动物侵害；然而，其潜在机制仍不清楚。多胺（PAs）在植物与昆虫的相互作用中起关键作用。在此，研究了硅在腐胺（Put）代谢中的作用及其在水稻对二化螟（SSB，Walker）抗性中的作用。结果表明，二化螟幼虫侵染导致水稻幼苗中游离腐胺大量积累。施用硅可增强水稻对二化螟的抗性，并抑制二化螟攻击诱导的腐胺积累，同时编码精氨酸脱羧酶（和）的腐胺生物合成基因的表达降低。此外，施用硅对创伤诱导的和的表达没有显著影响，但减弱了二化螟幼虫口腔分泌物诱导的和转录的进一步升高。同时，添加硅降低了受二化螟攻击植物中的腐胺和亚精胺含量。此外，外源施用腐胺减弱了硅增强的对二化螟幼虫的抗性，而精氨酸脱羧酶抑制剂外源D-精氨酸对水稻对二化螟的抗性表现出与硅类似的作用。我们的研究结果表明，硅提高水稻对二化螟的抗性，至少部分是通过减少食草刺激的腐胺积累实现的。
