Escalante-Beltrán Alina, Morales-Sandoval Pamela Helué, González-Astorga Claudia Berenice, Montoya-Martínez Amelia C, Cubedo-Ruiz Edgar A, Santoyo Gustavo, Parra-Cota Fannie Isela, de Los Santos-Villalobos Sergio
Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, Col. Centro, Cd. Obregón C.P. 8500, Sonora, Mexico.
Campo Experimental Norman E. Borlaug, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Norman E. Borlaug Km. 12, Cd. Obregón C.P. 85000, Sonora, Mexico.
Plants (Basel). 2025 Jul 7;14(13):2081. doi: 10.3390/plants14132081.
Strain 53B2 was isolated from a commercial maize ( L.) field located in the Yaqui Valley, Mexico. Its draft genome comprises 5,844,085 bp, with a G + C content of 37.5%, an N50 of 602,122 bp, an L50 of 4, and a total of 129 contigs. Genome-based taxonomic affiliation showed this strain belonged to . Genome annotation revealed 6394 coding DNA sequences (CDSs), organized into 332 subsystems. Among these, several CDSs were associated with traits relevant to plant growth promotion, including categories such as iron acquisition and metabolism (40 CDSs) and secondary metabolism (6 CDSs), among others. In vitro metabolic assays supported genomic predictions, confirming the strain's ability to produce IAA, solubilize phosphate, and tolerate abiotic stress. Additionally, greenhouse trials demonstrated that inoculation with 53B2 significantly enhanced plant growth parameters ( ≤ 0.05) versus uninoculated control: stem height increased by 22.8%, root length by 35.7%, stem and root fresh weights by 39.6% and 66.1%, and stem and root dry weights by 33.7% and 44.7%, respectively. This first report on the beneficial potential of 53B2 highlights its potential as a sustainable bioinoculant for maize cultivation.
菌株53B2是从墨西哥 Yaqui 谷地的一块商业化玉米（L.）田中分离出来的。其基因组草图包含5,844,085 bp，G + C含量为37.5%，N50为602,122 bp，L50为4，共有129个重叠群。基于基因组的分类归属表明该菌株属于 。基因组注释揭示了6394个编码DNA序列（CDS），分为332个子系统。其中，几个CDS与促进植物生长的性状相关，包括铁的获取和代谢（40个CDS）和次生代谢（6个CDS）等类别。体外代谢分析支持了基因组预测，证实了该菌株产生吲哚-3-乙酸（IAA）、溶解磷酸盐和耐受非生物胁迫的能力。此外，温室试验表明，与未接种对照相比，接种53B2显著提高了植物生长参数（P≤0.05）：茎高增加了22.8%，根长增加了35.7%，茎和根的鲜重分别增加了39.6%和66.1%，茎和根的干重分别增加了33.7%和44.7%。这份关于53B2有益潜力的首次报告突出了其作为玉米种植可持续生物接种剂的潜力。
