Katib Suphakorn, Apichai Sutasinee, Jiaranaikulwanitch Jutamas, Sirithunyalug Busaban, Ogata Fumihiko, Kawasaki Naohito, Grudpan Kate, Saenjum Chalermpong
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Research Center for Innovation in Analytical Science and Technology for Biodiversity Based Economic and Society (I-ANALY-S-T_B.BES-CMU), Chiang Mai University, Chiang Mai 50200, Thailand.
Molecules. 2025 Jun 28;30(13):2798. doi: 10.3390/molecules30132798.
A simple and rapid smartphone-based colorimetric assay for peracetic acid concentration was developed to facilitate point-of-use disinfectant preparations for infection prevention and control. The colorimetric detection was based on the oxidation of N,N-diethyl-phenylenediamine by peracetic acid through an intermediate reaction with potassium iodide, resulting in pink-magenta products. The colorimetric reaction was performed on a 96-well plate; then, the color products were photographed in one image. The color intensity was evaluated to determine the peracetic acid concentration using a custom-built mobile application named Modern Peracetic Acid Analysis. The relative green intensity of the pink-magenta products was directly proportional to the peracetic acid concentration in the range of 0.15 to 3.0 µg/mL. The detection and quantitation limits were 0.11 µg/mL and 0.34 µg/mL, respectively. The approach was successfully applied to determine the peracetic acid concentration in pharmaceutical disinfectant formulations. The results obtained using the proposed approach showed no significant differences from those obtained using acid-base titration at the 95% confidence level. The greenness of the proposed approach was evaluated using the Complementary Green Analytical Procedure Index, Analytical Greenness, and Blue Applicability Grade Index, demonstrating enhanced environmental friendliness and practical advantages, as well as simple, portable instrumentation that is easier to operate than traditional spectrophotometric and titration methods. Furthermore, a sustainability assessment based on the Need, Quality, and Sustainability index underscored its enhanced sustainability.
开发了一种基于智能手机的简单快速的过氧乙酸浓度比色测定法，以促进用于感染预防和控制的即用型消毒剂制备。比色检测基于过氧乙酸通过与碘化钾的中间反应氧化N,N-二乙基苯二胺，产生品红色产物。比色反应在96孔板上进行；然后，对显色产物拍摄一张图像。使用名为“现代过氧乙酸分析”的定制移动应用程序评估颜色强度，以确定过氧乙酸浓度。品红色产物的相对绿色强度在0.15至3.0 µg/mL范围内与过氧乙酸浓度成正比。检测限和定量限分别为0.11 µg/mL和0.34 µg/mL。该方法成功应用于测定药物消毒剂配方中的过氧乙酸浓度。在95%置信水平下，使用所提出方法获得的结果与使用酸碱滴定法获得的结果无显著差异。使用互补绿色分析程序指数、分析绿色度和蓝色适用性等级指数评估了所提出方法的绿色度，表明其具有更高的环境友好性和实际优势，以及比传统分光光度法和滴定法更易于操作的简单便携仪器。此外，基于需求、质量和可持续性指数的可持续性评估强调了其更高的可持续性。
