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DNA类型对DNA复合物薄层性质的影响。

DNA Type Influence on Properties of Thin Layer of DNA Complexes.

作者信息

Radko Aleksandra, Makyła-Juzak Katarzyna, Ekiert Robert, Chudzik Julia, Sokołowska Dagmara, Lalik Sebastian, Marzec Monika

机构信息

Institute of Physics, Jagiellonian University, Łojasiewicza 11, 30-348 Kraków, Poland.

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland.

出版信息

Materials (Basel). 2025 Jun 26;18(13):3022. doi: 10.3390/ma18133022.

Abstract

In the search for new functional materials, strong emphasis is placed on the ecological aspect, which is why thin layers of materials based on deoxyribonucleic acid (DNA) are fascinating from the point of view of applications. Thin layers of DNA-cationic surfactant complexes were created on mica slices using the Langmuir-Blodgett deposition technique. Three cationic surfactants (CTMA, BAC, HDP) and two types of DNA (linear dsDNA and plasmid pDNA) were used to synthesise the complexes. It was shown that the pattern of the obtained layer depended on the lifting conditions, type of DNA, and type of surfactant. The elongated structures that formed along the layer lifting direction were examined by AFM imaging and fast Fourier transform analysis. The main difference between the layers formed by plasmid pDNA-based and linear dsDNA-based complexes was the thickness of the stripes and the minimum surface pressures at which elongated structures were formed.

摘要

在寻找新型功能材料的过程中,人们非常重视生态方面,这就是为什么基于脱氧核糖核酸(DNA)的材料薄层从应用角度来看很有吸引力。使用朗缪尔-布洛杰特沉积技术在云母片上制备了DNA-阳离子表面活性剂复合物的薄层。使用三种阳离子表面活性剂(CTMA、BAC、HDP)和两种类型的DNA(线性双链DNA和质粒pDNA)来合成复合物。结果表明,所得层的图案取决于提拉条件、DNA类型和表面活性剂类型。通过原子力显微镜成像和快速傅里叶变换分析对沿层提拉方向形成的细长结构进行了研究。基于质粒pDNA的复合物和基于线性双链DNA的复合物形成的层之间的主要区别在于条纹的厚度以及形成细长结构时的最小表面压力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/20f0/12250794/9a769fee5971/materials-18-03022-g0A1.jpg

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