源自诱导型和沃顿胶间充质干细胞的外泌体促进癌细胞的衰老样特征和迁移。

Exosomes Derived from Induced and Wharton's Jelly-Derived Mesenchymal Stem Cells Promote Senescence-like Features and Migration in Cancer Cells.

作者信息

Ababneh Nidaa A, AlDiqs Razan, Nashwan Sura, Ismail Mohammad A, Barham Raghda, Alatoom Renata M, Nairat Fairouz, Gharandouq Mohammad H, Al-Qaisi Talal, Awidi Abdalla, Saleh Tareq

机构信息

Cell Therapy Center, The University of Jordan, Amman 11942, Jordan.

South Australian ImmunoGENomics Cancer Institute, Adelaide Medical School, University of Adelaide, Adelaide, SA 5000, Australia.

出版信息

Int J Mol Sci. 2025 Jun 26;26(13):6178. doi: 10.3390/ijms26136178.

DOI:10.3390/ijms26136178

PMID:40649957

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12249808/

Abstract

Mesenchymal stem cell-derived exosomes (MSC-Exos) play a key role in tissue repair, immune regulation, and cancer biology. Due to limitations in MSC expansion and source variability, interest has shifted to induced pluripotent stem cell-derived MSCs (iMSCs) as a promising alternative. This study compares effects of exosomes derived from iMSCs (iMSC-Exos) and Wharton's jelly MSCs (WJMSC-Exos) on MCF7 and A549 cancer cells. Both types of exosomes reduced MCF7 proliferation and induced a senescence-like state, rather than apoptosis, although the antiproliferative effect was transient in A549 cells. Notably, WJMSC-Exos promoted migration in both MCF7 and A549, whereas iMSC-Exos did not exhibit this effect. Overall, WJMSC-Exos had a more robust impact on cancer cell proliferation and migration. These findings highlight the diverse effects of exosomes on cancer and the development of a senescence-like state as an important response to Exos exposure. Moreover, these findings invite for more careful evaluation of the therapeutic role of iMSC-derived Exos.

摘要

间充质干细胞衍生的外泌体（MSC-Exos）在组织修复、免疫调节和癌症生物学中发挥着关键作用。由于间充质干细胞扩增的局限性和来源的变异性，人们的兴趣已转向诱导多能干细胞衍生的间充质干细胞（iMSCs），将其作为一种有前景的替代方案。本研究比较了iMSCs衍生的外泌体（iMSC-Exos）和沃顿胶间充质干细胞衍生的外泌体（WJMSC-Exos）对MCF7和A549癌细胞的影响。两种类型的外泌体均降低了MCF7的增殖并诱导了类似衰老的状态，而非凋亡，尽管对A549细胞的抗增殖作用是短暂的。值得注意的是，WJMSC-Exos促进了MCF7和A549细胞的迁移，而iMSC-Exos未表现出这种作用。总体而言，WJMSC-Exos对癌细胞增殖和迁移的影响更为显著。这些发现突出了外泌体对癌症的多种作用以及类似衰老状态的发展是对外泌体暴露的重要反应。此外，这些发现促使人们更仔细地评估iMSC衍生的外泌体的治疗作用。