Jiang Zhengwan, Zhang Wei, Zhu Can, Hu Zhiyuan, Xiang Xinjian, Cao Dongsheng, Wang Xianwen
Department of Plastic and Reconstructive, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, P. R. China.
School of Biomedical Engineering, Anhui Medical University, Hefei 230032, P. R. China.
ACS Nano. 2025 Jul 22;19(28):26170-26192. doi: 10.1021/acsnano.5c07686. Epub 2025 Jul 12.
Bacterial infections cause great difficulties in the healing process of wound repair and may worsen this condition, seriously jeopardizing human health. Gram-negative bacteria are more problematic sources of infection because of their outer membrane and endotoxins. In this study, copper telluride@sodium alginate-poly(vinyl alcohol) nanofiber/gauze (CuTe@SA-PVA NF/G) was constructed and applied to Gram-negative bacterium-infected wounds to achieve good anti-infective effects through the powerful antimicrobial mechanism of copper telluride (CuTe) nanozymes. CuTe nanoflowers with peroxidase-like (POD-like), oxidase-like (OXD-like), and glutathione peroxidase-like (GSH-Px-like) activities were synthesized via a simple one-pot method to combat bacterial survival by generating reactive oxygen species (ROS) and consuming GSH. The results of in vitro antimicrobial experiments confirmed that the killing effect of CuTe nanozymes on Gram-negative bacteria far exceeded that on Gram-positive bacteria and that they could effectively eradicate all kinds of Gram-negative bacteria and disrupt the existence of their biofilms. The results revealed that CuTe nanozymes damage Gram-negative bacteria by inhibiting bacterial dormancy, affecting iron metabolism, inhibiting flagellar motility, and decreasing the production of extracellular polysaccharide (EPS) and lipopolysaccharide (LPS). To further adapt to clinical applications, we used electrostatic spinning technology to prepare CuTe@SA-PVA NF/G wound dressings with good biosafety, which were applied to Gram-negative bacterium-infected wounds and pressure ulcer-infected sites. Animal experiments revealed that CuTe@SA-PVA NF/G has good anti-infection effects and promotes wound healing. The transcriptomics results further revealed that CuTe@SA-PVA NF/G promoted wound healing through potent antimicrobial activity, modulation of the inflammatory response, and stimulation of angiogenesis and cell proliferation. This study successfully developed a CuTe@SA-PVA NF/G wound dressing with specific killing effects on Gram-negative bacteria, providing a promising approach for the clinical treatment of specific Gram-negative bacterial infections.
细菌感染给伤口修复的愈合过程带来巨大困难，并可能使病情恶化，严重危及人类健康。革兰氏阴性菌因其外膜和内毒素而成为更具问题的感染源。在本研究中，构建了碲化铜@海藻酸钠-聚乙烯醇纳米纤维/纱布（CuTe@SA-PVA NF/G），并将其应用于革兰氏阴性菌感染的伤口，通过碲化铜（CuTe）纳米酶强大的抗菌机制实现良好的抗感染效果。通过简单的一锅法合成了具有过氧化物酶样（POD样）、氧化酶样（OXD样）和谷胱甘肽过氧化物酶样（GSH-Px样）活性的CuTe纳米花，通过产生活性氧（ROS）和消耗谷胱甘肽来对抗细菌存活。体外抗菌实验结果证实，CuTe纳米酶对革兰氏阴性菌的杀灭效果远远超过对革兰氏阳性菌的杀灭效果，并且它们可以有效根除各种革兰氏阴性菌并破坏其生物膜的存在。结果表明，CuTe纳米酶通过抑制细菌休眠、影响铁代谢、抑制鞭毛运动以及减少细胞外多糖（EPS）和脂多糖（LPS）的产生来损伤革兰氏阴性菌。为了进一步适应临床应用，我们使用静电纺丝技术制备了具有良好生物安全性的CuTe@SA-PVA NF/G伤口敷料，并将其应用于革兰氏阴性菌感染的伤口和压疮感染部位。动物实验表明，CuTe@SA-PVA NF/G具有良好的抗感染效果并促进伤口愈合。转录组学结果进一步表明，CuTe@SA-PVA NF/G通过强大的抗菌活性、调节炎症反应以及刺激血管生成和细胞增殖来促进伤口愈合。本研究成功开发了一种对革兰氏阴性菌具有特异性杀灭作用的CuTe@SA-PVA NF/G伤口敷料，为临床治疗特定的革兰氏阴性菌感染提供了一种有前景的方法。
