用于无菌过滤的微孔过滤器可能会浸出影响细胞培养（HL 60）的有毒化合物。

Micropore filters for sterile filtration may leach toxic compounds affecting cell cultures (HL 60).

作者信息

Kristensen T, Mortensen B T, Nissen N I

出版信息

Exp Hematol. 1985 Dec;13(11):1188-91.

Abstract

Reduction in HL-60 cell growth could be traced unexpectedly to sterile filtration procedures. To circumvent this problem, 11 available brands of micropore filters (five prepacked and six to be packed and autoclaved) were investigated with the aim of finding the least toxic product. Samples of 10 ml of RPMI 1640 medium with 10% fetal calf serum added were filtered through four filters of each brand to detect even small amounts of leached toxic compounds. HL-60 cells were cultured in these filtered media for three days and the results compared with cultures using unfiltered medium. A large variation in growth inhibition was found between the filters investigated, ranging from 0% to about 90%. The growth inhibition was due to leaching of toxic compounds, as revealed by viability test, reseeding, and direct microscopy. Adsorption of essential proteins to the micropore filters was found for only one of the filters investigated in this study.

摘要

HL-60细胞生长的减少意外地可追溯到无菌过滤程序。为了规避这个问题，对11个可用品牌的微孔过滤器（5个预包装的和6个待包装并高压灭菌的）进行了研究，目的是找到毒性最小的产品。向10毫升添加了10%胎牛血清的RPMI 1640培养基样品通过每个品牌的四个过滤器进行过滤，以检测即使是少量的浸出有毒化合物。将HL-60细胞在这些过滤后的培养基中培养三天，并将结果与使用未过滤培养基的培养物进行比较。在所研究的过滤器之间发现生长抑制存在很大差异，范围从0%到约90%。活力测试、重新接种和直接显微镜检查表明，生长抑制是由于有毒化合物的浸出。在本研究中所研究的过滤器中，仅发现一种过滤器会吸附必需蛋白质。