Engel F
J Pharm Pharmacol. 1982 May;34(5):283-6. doi: 10.1111/j.2042-7158.1982.tb04708.x.
The use of membrane filters for filtration of serum-containing culture media can change a medium in such a way that cells cultured therein attain a significantly higher lactate-glucose metabolism than the same type of cells cultured in unfiltered medium. Five different filertypes were examined, but the response was only found after filtration with MF-filters of Millipore (mixed esters of cellulose). Furthermore, in two of the three cell lines examined minor growth inhibitions were also observed. The MF-filters were found to contain a compound bound to the filter material so that serum-containing medium, but not serum-free medium, or water, could extract it in amounts sufficient for metabolic - and growth effects. The compound in MF-filters is closely related to Triton-X-100 both with respect to u.v.-spectra and to biological effects.
使用膜过滤器过滤含血清的培养基会改变培养基，使得在此培养基中培养的细胞比在未过滤培养基中培养的同种细胞具有显著更高的乳酸 - 葡萄糖代谢。研究了五种不同类型的过滤器，但仅在用密理博（纤维素混合酯）的MF过滤器过滤后才观察到这种反应。此外，在所检测的三种细胞系中的两种中还观察到了轻微的生长抑制。发现MF过滤器含有一种与滤材结合的化合物，使得含血清的培养基（而非无血清培养基或水）能够提取出足以产生代谢和生长效应的量。MF过滤器中的化合物在紫外光谱和生物学效应方面都与曲拉通X - 100密切相关。
