解淀粉芽孢杆菌AAHM-BV2354对提高苏氏圆腹芒（Pangasianodon hypophthalmus）免疫力、生长性能、抗氧化活性及抗迟缓爱德华氏菌感染的益生菌和后生元效应

Probiotic and postbiotic effects of Bacillus velezensis AAHM-BV2354 on boosting immunity, growth performance, antioxidant activity and resistance to Edwardsiella tarda infection in pangasius (Pangasianodon hypophthalmus).

作者信息

Wiratama Nugroho, Kumwan Benchawan, Meachasompop Pakapon, Adisornprasert Yosapon, Srisapoome Prapansak, Thompson Kim D, Phrompanya Phornphan, Thangsunan Patcharapong, Thangsunan Pattanapong, Saenphet Kanokporn, Saenphet Supap, Buncharoen Wararut, Uchuwittayakul Anurak

机构信息

Laboratory of Aquatic Animal Health Management, Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; Center of Excellence in Aquatic Animal Health Management, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; PT. Central Proteinaprima, Tbk, Treasury Tower Lt. 8, District 8 SCBD Lot. 28, Senayan, Jakarta, 12190, Indonesia.

出版信息

Fish Shellfish Immunol. 2025 Oct;165:110561. doi: 10.1016/j.fsi.2025.110561. Epub 2025 Jul 11.

DOI:10.1016/j.fsi.2025.110561

PMID:40653180

Abstract

The increasing demand for Pangasianodon hypophthalmus underscores the importance of addressing disease-related productivity challenges. Probiotic feed additives present a promising approach for improving disease management, enhancing productivity, and promoting sustainable growth in pangasius farming. This study investigated the impact of dietary supplementation with probiotics, postbiotics, and their synergistic combination, utilising Bacillus velezensis AAHM-BV2354, on fish health, growth performance, oxidative stress status, innate immune responses and disease resistance in pangasius, aimed at preventing Edwardsiella tarda infection. The experimental design featured five treatment groups, each receiving feed coated with different components of Bacillus velezensis AAHM-BV2354, including cells (BV_Cell), cell-free supernatant (BV_Cfs), and a combination of both (BV_Cell + Cfs), with 2 type of control group were feed top-dressed with 100 mL of 0.85 % NaCl/kg diet (Control) and feed coated with 100 mL of TSB medium/kg diet (C_TSB) for 30 days before the challenge test with Edwardsiella tarda. The findings revealed that the BV_Cfs treatment group exhibited a notable enhancement in SGR, ADG, and upregulation gh1 gene. Bacillus velezensis AAHM-BV2354 demonstrated protective effects by reducing oxidative stress in MDA levels and boosting antioxidant enzymes in specific organs. Gene expression analysis revealed an upregulation in the expression of immune genes (lygl1, tgfb, tnf, b2ml), proinflammatory genes (litaf, ifngr1l, c3, il13, il1b), and antioxidant genes (cusr, cat, gpx3, hspa13, nos1) in various fish organs after administering probiotics, postbiotics, and their synergistic combination of Bacillus velezensis AAHM-BV2354. The subsequent challenge test with E. tarda showed that the BV_Cell treatment achieved the highest survival rate (90.62 %). These results indicate that all bioactive components of B. velezensis AAHM-BV2354 can serve as natural immunostimulants, enhancing the health and growth of pangasius.

摘要

对苏氏圆腹芒需求的不断增加凸显了解决与疾病相关的生产力挑战的重要性。益生菌饲料添加剂为改善疾病管理、提高生产力和促进苏氏圆腹芒养殖的可持续增长提供了一种有前景的方法。本研究调查了使用解淀粉芽孢杆菌AAHM-BV2354对益生菌、后生元及其协同组合进行日粮补充，对苏氏圆腹芒的鱼类健康、生长性能、氧化应激状态、先天免疫反应和抗病性的影响，旨在预防迟缓爱德华氏菌感染。实验设计包括五个处理组，每组接受涂有解淀粉芽孢杆菌AAHM-BV2354不同成分的饲料，包括细胞（BV_Cell）、无细胞上清液（BV_Cfs）以及两者的组合（BV_Cell + Cfs），有2个对照组，在迟缓爱德华氏菌攻毒试验前30天，分别用100 mL 0.85% NaCl/kg日粮进行表层添加饲喂（对照）和用100 mL胰蛋白胨大豆肉汤培养基/kg日粮进行涂覆饲喂（C_TSB）。研究结果显示，BV_Cfs处理组的特定生长率（SGR）、平均日增重（ADG）显著提高，gh1基因上调。解淀粉芽孢杆菌AAHM-BV2354通过降低丙二醛（MDA）水平的氧化应激和提高特定器官中的抗氧化酶，表现出保护作用。基因表达分析显示，在施用益生菌、后生元及其解淀粉芽孢杆菌AAHM-BV2354的协同组合后，各种鱼类器官中的免疫基因（lygl1、tgfb、tnf、b2ml）、促炎基因（litaf、ifngr1l、c3、il13、il1b）和抗氧化基因（cusr、cat、gpx3、hspa13、nos1）的表达上调。随后的迟缓爱德华氏菌攻毒试验表明，BV_Cell处理组的存活率最高（90.62%）。这些结果表明，解淀粉芽孢杆菌AAHM-BV2354的所有生物活性成分都可作为天然免疫刺激剂，增强苏氏圆腹芒的健康和生长。