Lehninger A L, Reynafarje B, Hendler R W, Shrager R I
FEBS Lett. 1985 Nov 18;192(2):173-8. doi: 10.1016/0014-5793(85)80102-2.
Costa, L.E., Reynafarje, B. and Lehninger, A.L. [(1984) J. Biol. Chem. 259, 4802-4811] have reported 'second-generation' measurements of the H+/O ratio approaching 8.0 for vectorial H+ translocation coupled to succinate oxidation by rat liver mitochondria. In a Commentary in this Journal [Krab, K., Soos, J. and Wikström, M. (1984) FEBS Lett. 178, 187-192] it was concluded that the measurements of Costa et al. significantly overestimated the true H+/O stoichiometry. It is shown here that the mathematical simulation on which Krab et al. based this claim is faulty and that data reported by Costa et al. had already excluded the criticism advanced by Krab et al. Also reported are new data, obtained under conditions in which the arguments of Krab et al. are irrelevant, which confirm that the H+/O ratio for succinate oxidation extrapolated to level flow is close to 8.
科斯塔，L.E.，雷纳法尔耶，B.和莱宁格，A.L. [（1984年）《生物化学杂志》259卷，4802 - 4811页] 报告了 “第二代” H⁺/O 比率的测量结果，对于大鼠肝线粒体中与琥珀酸氧化偶联的向量型 H⁺ 转运，该比率接近8.0。在本杂志的一篇评论文章中 [克拉布，K.，苏斯，J.和维克斯特伦，M. （1984年）《欧洲生物化学学会联合会快报》178卷，187 - 192页] 得出结论，科斯塔等人的测量结果显著高估了真实的H⁺/O 化学计量比。本文表明，克拉布等人提出这一主张所依据的数学模拟是有缺陷的，而且科斯塔等人报告的数据已经排除了克拉布等人提出的批评。还报告了在克拉布等人的观点不相关的条件下获得的新数据，这些数据证实，外推至水平流时琥珀酸氧化的H⁺/O 比率接近8。
