Li Yan, Zhong Jian-Yuan, Mo Ya-Qi, Qin Li-Mei, Aschner Michael, Jiang Yue-Ming
Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease, Nanning, 530021, China.
Key Laboratory of Prevention and Control of Highly Prevalent Diseases in Guangxi Colleges and Universities, School of Public Health, Guangxi Medical University, Nanning, 530021, China.
Anal Biochem. 2025 Nov;706:115941. doi: 10.1016/j.ab.2025.115941. Epub 2025 Jul 11.
The content of elements in the body has a significant relationship with human health. However, the wide variation in the concentrations of different elements in blood or urine poses challenges for accurate detection. This study presents a dual-mode ICP-MS methodology integrating kinetic energy discrimination (KED) and dynamic reaction cell (DRC) technologies, specifically designed to address the analytical challenge of quantifying 22 physiologically critical elements (Be, V, Cr, Mn, Fe, Ca, Mg, Ba, Co, Cd, Cu, Zn, As, Se, Ti, Sr, Ni, Mo, Sn, Sb, Tl, Pb) spanning six orders of magnitude in concentration (μg/L to g/L) within complex biological matrices. Rejection parameter q (RPq) adjusts the low-mass cutoff, while rejection parameter a (RPa) controls the high-mass cutoff. Optimizing both extends the low-interference dynamic range. The method's core innovation lies in the synchronized optimization of quadrupole parameters RPa and RPq, which enables simultaneous suppression of matrix-derived polyatomic interferences while maintaining linear detector response across ultra-wide dynamic ranges. The method employs a dual-mode analysis approach: (1) In urine analysis, the KED-He mode is suitable for analyzing all elements. (2) In whole blood analysis, DRC-NH mode is used for interference-prone elements (Mn, Cr, Ca), and KED-He mode is used for the remaining elements. Strategic optimization of quadrupole rejection parameters (RPa/RPq) achieved intra-day precision of 0.5-7.2 % (blood) and 1.6-5.5 % (urine), with inter-day variations ≤9.6 %, demonstrating robust performance for multi-element profiling across clinically relevant concentration ranges.
人体内元素的含量与人体健康有着重要关系。然而，血液或尿液中不同元素浓度的广泛差异给准确检测带来了挑战。本研究提出了一种结合动能歧视（KED）和动态反应池（DRC）技术的双模式电感耦合等离子体质谱方法，专门用于应对在复杂生物基质中对22种生理关键元素（铍、钒、铬、锰、铁、钙、镁、钡、钴、镉、铜、锌、砷、硒、钛、锶、镍、钼、锡、锑、铊、铅）进行定量分析的挑战，这些元素的浓度范围跨越六个数量级（μg/L至g/L）。排斥参数q（RPq）调整低质量截止值，而排斥参数a（RPa）控制高质量截止值。对两者进行优化可扩展低干扰动态范围。该方法的核心创新在于对四极杆参数RPa和RPq的同步优化，这使得在超宽动态范围内同时抑制基质衍生的多原子干扰，同时保持线性探测器响应。该方法采用双模式分析方法：（1）在尿液分析中，KED-He模式适用于分析所有元素。（2）在全血分析中，DRC-NH模式用于易受干扰的元素（锰、铬、钙），而KED-He模式用于其余元素。四极杆排斥参数（RPa/RPq）的策略性优化实现了日内精密度为0.5 - 7.2%（血液）和1.6 - 5.5%（尿液），日间变化≤9.6%，证明了在临床相关浓度范围内进行多元素分析时具有强大的性能。
