Xing Binglin, Shan Lin, Liu Chunyu, Wang Zhenglei, Li Tianzhu, Wang Jing, Cheng Yichen, Yang Jing, Guo Shengya, Li Jiamei, Xu Duo, Du Bo, Yang Lei
State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Sanya Nanfan Research Institute, Hainan University, Sanya 572024, China.
J Agric Food Chem. 2025 Aug 27;73(34):21666-21680. doi: 10.1021/acs.jafc.5c03346. Epub 2025 Jul 13.
Parasitoids are crucial natural enemies of , a globally notorious pest. Upon parasitization, host metabolism and immune responses were profoundly disrupted, thereby favoring the development of wasps offsprings. However, the underlying regulatory mechanisms remain elusive. In this study, transcriptome sequencing was utilized to investigate the host metabolic and immune response changes at 4 h, 12 h, and 24 h postparasitization by sp.2. We identified 4070 differentially expressed genes, enriched in metabolic (e.g., tricarboxylic acid cycle, insulin synthesis) and immune (e.g., Toll/Imd pathways) pathways, and RT-qPCR further validated core gene expressions involved in these pathways. Physiological analyses revealed that sp.2 parasitism altered the host larvae's protein, lipid, and sugar content, inhibited hemocyte spreading, increased hemocyte mortality, and suppressed hemolymph melanization. Collectively, this study sheds light on the host metabolic and immune modulation by sp.2 parasitism and provides a novel insight into the mechanisms underpinning their interactions. This discovery lays a solid foundation for the practical application of parasitoids in biological control programs.
寄生蜂是一种全球臭名昭著的害虫的重要天敌。被寄生后，宿主的新陈代谢和免疫反应会受到严重干扰，从而有利于黄蜂后代的发育。然而，其潜在的调控机制仍不清楚。在本研究中，利用转录组测序来研究在被sp.2寄生后4小时、12小时和24小时宿主的代谢和免疫反应变化。我们鉴定出4070个差异表达基因，这些基因富集于代谢（如三羧酸循环、胰岛素合成）和免疫（如Toll/Imd途径）途径，并且RT-qPCR进一步验证了这些途径中涉及的核心基因表达。生理分析表明，sp.2寄生改变了宿主幼虫的蛋白质、脂质和糖含量，抑制了血细胞铺展，增加了血细胞死亡率，并抑制了血淋巴黑化。总的来说，本研究揭示了sp.2寄生对宿主代谢和免疫的调节作用，并为它们相互作用的潜在机制提供了新的见解。这一发现为寄生蜂在生物防治计划中的实际应用奠定了坚实的基础。
