Atterwill C K, Atkinson D J
Mol Cell Endocrinol. 1985 Nov;43(1):105-10. doi: 10.1016/0303-7207(85)90047-4.
In neurone-enriched cultures derived from early postnatal rat cerebellum and maintained in serum-free medium, addition of serum (10% FCS) evoked a rapid increase in Na+ pump activity (as measured by ouabain-sensitive 86Rb accumulation) by activation of a Na+/H+ exchanger. This effect did not occur with cultured cerebellar astrocytes. In contrast, exposure to serum increased DNA synthesis ([3H]thymidine incorporation) in both cultured cerebellar astrocytes and in the neurone-enriched cultures. However, in the latter cultures this effect was shown by autoradiography to be due to contaminating astrocytes. Thus, in cultured cerebellar neural cells an enhancement of intracellular Na+ accumulation by serum factors may not be linked to initiation of DNA synthesis. Furthermore, raising intracellular Na+ by ouabain exposure actually decreased neural cellular DNA synthesis.
在源自新生大鼠小脑早期且维持于无血清培养基中的富含神经元的培养物中，添加血清（10%胎牛血清）通过激活钠氢交换体使钠泵活性迅速增加（通过哇巴因敏感的86Rb摄取来测量）。这种效应在培养的小脑星形胶质细胞中未出现。相反，血清暴露增加了培养的小脑星形胶质细胞和富含神经元的培养物中的DNA合成（[3H]胸苷掺入）。然而，在后者的培养物中，放射自显影显示这种效应是由于污染的星形胶质细胞所致。因此，在培养的小脑神经细胞中，血清因子增强细胞内钠积累可能与DNA合成起始无关。此外，通过哇巴因暴露提高细胞内钠实际上会降低神经细胞的DNA合成。
