组织样本制备方法对髓鞘敏感定量磁共振成像的影响。
Impact of Tissue Sample Preparation Methods on Myelin-Sensitive Quantitative MR Imaging.
作者信息
Murguia Amaya, Swanson Scott D, Scheven Ulrich, Jacobson Andrea, Nielsen Jon-Fredrik, Fessler Jeffrey A, Seraji-Bozorgzad Navid
机构信息
Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI, USA.
Department of Radiology, Michigan Medicine, University of Michigan, Ann Arbor, MI, USA.
出版信息
bioRxiv. 2025 May 10:2025.05.05.652075. doi: 10.1101/2025.05.05.652075.
PURPOSE
Validation of quantitative MRI (qMRI) parameters with histology is often done with ex vivo fixed tissue samples. Freezing is another common form of tissue preservation, but the effects of freezing and thawing tissue on myelin-sensitive quantitative MRI parameters and their correlation with histology require further analysis.
METHODS
Myelin water imaging, off-resonance RF saturation magnetization transfer (MT), and selective inversion recovery MT MRI experiments were conducted on 14 fresh, thawed, and fixed sheep brain tissue samples to calculate various surrogate measures of myelin content. These measures were compared with luxol fast blue (LFB) histological stain results.
RESULTS
Fresh, thawed, and fixed tissue qMRI values correlated well with LFB. Thawed and fixed tissue exhibited modest increases, between 3-32%, for most qMRI parameter values compared to fresh. Histology results showed that thawed samples did not lose tissue integrity from the freezing process.
CONCLUSION
Freezing is a reasonable alternative tissue preservation method to fixation for use in qMRI analysis, but may differentially affect qMRI parameter values in regions with varying myelin content.
目的
定量磁共振成像（qMRI）参数与组织学的验证通常使用离体固定组织样本进行。冷冻是另一种常见的组织保存形式，但冷冻和解冻组织对髓鞘敏感的定量MRI参数的影响及其与组织学的相关性需要进一步分析。
方法
对14个新鲜、解冻和固定的绵羊脑组织样本进行髓鞘水成像、非共振射频饱和磁化传递（MT）和选择性反转恢复MT MRI实验，以计算各种髓鞘含量的替代指标。将这些指标与Luxol固蓝（LFB）组织学染色结果进行比较。
结果
新鲜、解冻和固定组织的qMRI值与LFB相关性良好。与新鲜组织相比，解冻和固定组织的大多数qMRI参数值有适度增加，增幅在3%至32%之间。组织学结果表明，解冻后的样本在冷冻过程中没有丧失组织完整性。
结论
冷冻是一种用于qMRI分析的合理替代固定的组织保存方法，但可能对髓鞘含量不同的区域的qMRI参数值产生不同影响。
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