Horáková A, Horák J, Tomajková E
Gegenbaurs Morphol Jahrb. 1985;131(4):477-92.
The neurons of the pars caudalis nuclei tuberomammillaris (pc-NTM) were studed light-microscopically and electron-microscopically in sheep and rams of Merino breed. In our study we observed: In the regarded neural nucleus, there is the majority of the great neurons (up to 60 microns in diameter) rich in the NISSL's bodies. When stained with the cresyl violet, the NISSL's substance is apparently stored mainly in peripheral area of the cell body and in the distant parts of numerous protoplasmic processes, what evokes an impression of the "jagged" surface of these cells. After staining with paraldehyde fuchsin, we found purple coloured lumps of irregular shape stored outside the cell bodies, in the neuropil. The less extended cells, usually with lower content of NISSL bodies, are in pc-NTM less frequent. In the electron-microscopic study we identified 3 types of neurons: Cells rich in rough endoplasmic reticulum; "light" cells, "dark" cells. The cells of the 1st type were the most frequent ones. Cisterns of rough endoplasmic reticulum in the 1st type of cells are often dilated. The protoplasmic processes of these cells are frequently stepped over by flat tubuli of endoplasmic reticulum. The 2nd type of cells is characterized by the light cytoplasmic matrix, low quantity of endoplasmic reticulum and frequent occurrence of lipofuscin bodies. The 3rd type of cells are characterized by the high density of cytoplasmic matrix, well developed GOLGI complex, and very broad cisterns of endoplasmic reticulum, forming a labyrinth, and it is bound to a broad perinuclear space.
对美利奴品种绵羊和公羊的乳头体尾侧核(pc-NTM)神经元进行了光镜和电镜研究。在我们的研究中观察到:在所研究的神经核中,大多数大神经元(直径达60微米)富含尼氏体。用甲酚紫染色时,尼氏体物质显然主要储存在细胞体的周边区域以及众多原生质突起的远端部分,这给人一种这些细胞“锯齿状”表面的印象。用多聚甲醛-品红染色后,我们在神经毡中发现细胞体外部有紫色的不规则块状物。延伸较少的细胞,通常尼氏体含量较低,在pc-NTM中较少见。在电镜研究中,我们鉴定出3种类型的神经元:富含粗面内质网的细胞;“亮”细胞、“暗”细胞。第一类细胞最为常见。第一类细胞中的粗面内质网池常常扩张。这些细胞的原生质突起经常被内质网的扁平小管跨过。第二类细胞的特征是细胞质基质浅、内质网数量少且脂褐素体常见。第三类细胞的特征是细胞质基质密度高、高尔基体发达以及内质网池非常宽阔,形成一个迷宫,并且与宽阔的核周间隙相连。