Meena Manoj, Subramaniam Tulsi, Nikalje Monali R, Karthiyeyan Bhuvaneshwari, Vihan Arjit, Devi Sagolshem D
Department of Oral Medicine and Radiology, Rajasthan Dental College and Hospital, Jaipur, Rajasthan, India.
Department of Dentistry Symbiosis Medical College for Women, Symbiosis International (Deemed University), Pune, Maharashtra, India.
J Pharm Bioallied Sci. 2025 Jun;17(Suppl 2):S1730-S1732. doi: 10.4103/jpbs.jpbs_289_25. Epub 2025 Jun 18.
Oral systemic illnesses are associated with other systemic health issues, such as cardiovascular diseases, diabetes, and autoimmune disorders. The discovery of dependable biomarkers is crucial for the early diagnosis and monitoring of these illnesses. The identification of non-invasive blood biomarkers has emerged as a viable diagnostic method, providing improved patient compliance and reduced procedure risks. This research seeks to assess the efficacy of certain non-invasive biomarkers in the diagnosis and monitoring of oral systemic illnesses.
A total of 100 individuals (50 with clinically confirmed oral systemic illnesses and 50 healthy controls) were recruited from a tertiary care facility. Non-invasive peripheral blood samples were obtained with a micro-collection device. Biomarkers such as C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) were evaluated by enzyme-linked immunosorbent assay (ELISA). Statistical study used ANOVA and regression models to ascertain the relevance and association between biomarker levels and illness severity.
The concentrations of CRP, IL-6, and TNF-α were markedly increased in patients with oral systemic illnesses compared to healthy controls (CRP: 10.2 ± 2.1 mg/L vs. 2.5 ± 0.8 mg/L, < 0.01; IL-6: 8.7 ± 1.5 pg/mL vs. 3.2 ± 0.9 pg/mL, < 0.01; TNF-α: 12.4 ± 2.0 pg/mL vs. 5.1 ± 1.3 pg/mL, < 0.01). Regression analysis revealed a robust positive connection between IL-6 and illness severity (r = 0.82, < 0.01). The sensitivity and specificity of the combined biomarker detection were 92% and 88%, respectively.
Non-invasive detection of blood biomarkers has considerable promise in diagnosing and managing oral systemic illnesses. Increased concentrations of CRP, IL-6, and TNF-α are dependable markers of illness existence and intensity.
口腔系统性疾病与其他全身健康问题相关，如心血管疾病、糖尿病和自身免疫性疾病。发现可靠的生物标志物对于这些疾病的早期诊断和监测至关重要。非侵入性血液生物标志物的识别已成为一种可行的诊断方法，可提高患者的依从性并降低操作风险。本研究旨在评估某些非侵入性生物标志物在口腔系统性疾病诊断和监测中的功效。
从一家三级医疗机构招募了总共100名个体（50名临床确诊的口腔系统性疾病患者和50名健康对照）。使用微量采集装置采集非侵入性外周血样本。通过酶联免疫吸附测定（ELISA）评估生物标志物，如C反应蛋白（CRP）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。统计研究使用方差分析和回归模型来确定生物标志物水平与疾病严重程度之间的相关性和关联性。
与健康对照相比，口腔系统性疾病患者的CRP、IL-6和TNF-α浓度显著升高（CRP：10.2±2.1mg/L对2.5±0.8mg/L，<0.01；IL-6：8.7±1.5pg/mL对3.2±0.9pg/mL，<0.01；TNF-α：12.4±2.0pg/mL对5.1±1.3pg/mL，<0.01）。回归分析显示IL-6与疾病严重程度之间存在强烈的正相关（r = 0.82，<0.01）。联合生物标志物检测的敏感性和特异性分别为92%和88%。
血液生物标志物的非侵入性检测在口腔系统性疾病的诊断和管理中具有很大的前景。CRP、IL-6和TNF-α浓度升高是疾病存在和严重程度的可靠标志物。
