Matsuura F, Jones M Z
J Biol Chem. 1985 Dec 5;260(28):15239-45.
Four oligosaccharide fractions were isolated and purified from the kidney of goats affected with beta-mannosidosis by repeating Bio-Gel P-2 column chromatography. The structural characterization of the purified oligosaccharide fractions (oligosaccharides A, B, C1,2, and D) included sugar composition analysis by gas chromatography, sugar sequence analysis by mass spectrometry of their permethylated alditols, and by methylation analysis as well as anomeric configuration studies by exoglycosidase digestions. Oligosaccharides A and B were the major oligosaccharides accumulating in the kidney and were elucidated as Man beta 1-4GlcNAc and Man beta 1-4GlcNAc beta 1-4GlcNAc, respectively (Matsuura, F., Laine, R. A., and Jones, M. Z. (1981) Arch. Biochem. Biophys. 211, 485-493). Oligosaccharide C1,2 was a mixture of two tetrasaccharides and oligosaccharide D was a pentasaccharide. The proposed structures are: oligosaccharide C1, Man beta 1-4GlcNAc beta 1-4Man beta 1-4GlcNAc; oligosaccharide C2, Man alpha 1-6Man beta 1-4GlcNAc beta 1-4GlcNAc; oligosaccharide D, Man beta 1-4GlcNAc beta 1-4Man beta 1-4GlcNAc beta 1-4GlcNAc. Tetrasaccharide C1 and pentasaccharide D are heretofore undiscovered oligosaccharides. There is no precedent for these structures in glycoproteins or other glycoconjugates. One possibility which accounts for the presence of oligosaccharide C1 and D is that a bisecting N-acetylglucosamine (the beta-N-acetylglucosamine residue linked at the C-4 position of the beta-mannosyl residue of the trimannosyl core of the asparagine-linked sugar chains) is linked by a beta-mannosyl residue. Moreover, the detection of oligosaccharides containing two N-acetylglucosamine residues at the reducing terminus, together with those containing a single N-acetylglucosamine residue, is further corroboration of species-specific differences in glycoprotein catabolic pathways (Hancock, L. W., and Dawson, G. (1984) Fed. Proc. 43, 1552) or in glycoprotein structures.
通过重复使用Bio-Gel P-2柱色谱法，从患有β-甘露糖苷病的山羊肾脏中分离并纯化出四个寡糖级分。对纯化后的寡糖级分（寡糖A、B、C1、2和D）的结构表征包括：通过气相色谱法进行糖组成分析，通过对其全甲基化糖醇进行质谱分析以及甲基化分析来进行糖序列分析，还通过外切糖苷酶消化进行端基构型研究。寡糖A和B是在肾脏中积累的主要寡糖，分别被鉴定为Manβ1-4GlcNAc和Manβ1-4GlcNAcβ1-4GlcNAc（松浦，F.，莱恩，R. A.，和琼斯，M. Z.（1981年）《生物化学与生物物理学文献》211，485 - 493）。寡糖C1,2是两种四糖的混合物，寡糖D是一种五糖。推测的结构如下：寡糖C1，Manβ1-4GlcNAcβ1-4Manβ1-4GlcNAc；寡糖C2，Manα1-6Manβ1-4GlcNAcβ1-4GlcNAc；寡糖D，Manβ1-4GlcNAcβ1-4Manβ1-4GlcNAcβ1-4GlcNAc。四糖C1和五糖D是迄今未发现的寡糖。在糖蛋白或其他糖缀合物中没有这些结构的先例。解释寡糖C1和D存在的一种可能性是，一个平分型N-乙酰葡糖胺（连接在天冬酰胺连接糖链的三甘露糖核心的β-甘露糖基残基的C-4位置的β-N-乙酰葡糖胺残基）通过一个β-甘露糖基残基连接。此外，在还原端检测到含有两个N-乙酰葡糖胺残基的寡糖以及含有单个N-乙酰葡糖胺残基的寡糖，进一步证实了糖蛋白分解代谢途径（汉考克，L. W.，和道森，G.（1984年）《联邦会议录》43，1552）或糖蛋白结构中的物种特异性差异。
