Sinibaldi R M, Turpen T
J Biol Chem. 1985 Dec 15;260(29):15382-5.
A temperature shift from 25 to 41 degrees C initiates the synthesis of a specific set of proteins in maize, including a peptide of 60 kilodaltons. Using an in vitro mitochondrial protein synthesizing system, we provide evidence that this 60-kDa heat shock protein is encoded within the organelle. Further support for this heat-induced protein being encoded within mitochondria is that its synthesis is inhibited in whole seedlings by chloramphenicol. This 60-kDa heat shock protein is induced in all lines of maize we examined. Additionally, a heat-induced peptide of similar size (62 kDa) can be detected in isolated mitochondria of a dicot plant, Brassica campestris. The function of the heat shock protein encoded within the mitochondria remains unknown.
温度从25摄氏度升至41摄氏度会引发玉米中一组特定蛋白质的合成，其中包括一种60千道尔顿的肽。利用体外线粒体蛋白质合成系统，我们提供了证据表明这种60千道尔顿的热休克蛋白是由细胞器内编码的。这种热诱导蛋白由线粒体内编码的进一步证据是，其合成在全株幼苗中受到氯霉素的抑制。我们检测的所有玉米品系中都会诱导产生这种60千道尔顿的热休克蛋白。此外，在双子叶植物油菜的分离线粒体中可以检测到一种大小相似（62千道尔顿）的热诱导肽。线粒体内编码的热休克蛋白的功能仍然未知。
