Nieto-Sotelo J, Ho T H
J Biol Chem. 1987 Sep 5;262(25):12288-92.
Examination of the proteins synthesized by isolated mitochondria, chloroplasts, or proplastids from maize tissues showed that a heat treatment at 40 degrees C does not induce or enhance the synthesis of any protein when compared to preparations treated at the control temperature of 28 degrees C. These observations are consistent with the results obtained by labeling proteins in vivo under sterile conditions. In vivo labeling in the presence of cycloheximide during heat shock showed no heat shock protein synthesis. Labeling in the presence of chloramphenicol during heat shock showed a similar heat shock protein pattern as in the absence of the inhibitor. It is concluded that maize organelles do not synthesize heat shock proteins and that, if present, they may be due to bacterial contamination.
对从玉米组织中分离出的线粒体、叶绿体或前质体合成的蛋白质进行检测发现，与在28℃对照温度下处理的样品相比，40℃热处理不会诱导或增强任何蛋白质的合成。这些观察结果与在无菌条件下对体内蛋白质进行标记所获得的结果一致。在热休克期间存在环己酰亚胺的情况下进行体内标记，未显示热休克蛋白合成。在热休克期间存在氯霉素的情况下进行标记，显示出与不存在抑制剂时相似的热休克蛋白模式。得出的结论是，玉米细胞器不会合成热休克蛋白，并且如果存在热休克蛋白，可能是由于细菌污染所致。
