Yu Jun, Jin Shengbo, Piao Haozhe, Li Mingzhu
Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, 110847, P.R. China.
College of Acupuncture and Massage of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, 110847, P.R. China.
Open Life Sci. 2025 Jul 8;20(1):20251122. doi: 10.1515/biol-2025-1122. eCollection 2025.
The aim of this study is to systematically retrieve, synthesize, and assess methodologies employed in the development of paclitaxel-induced peripheral neuropathic pain (PIPNP) animal models, with the objective of optimizing protocols for model construction and evaluation. A structured search strategy was implemented using the terms "Paclitaxel-induced peripheral neuropathic pain" OR "Chemotherapy-induced peripheral neuropathic pain" AND "animal models" OR "rats" OR "mice" OR "experimental animals" across the China National Knowledge Infrastructure, Wanfang, and VIP databases. Identical search criteria were applied to the PubMed and Web of Science platforms. The literature search covered publications from database inception through December 31, 2024. A total of 128 articles were reviewed, including 18 in Chinese and 110 in English. All studies employed rodent models, including 16 strains and including both sexes, with ages ranging from 5 weeks to 10 months. In rats, 28 distinct modeling protocols were identified: 4 involving continuous paclitaxel injections, 23 employing alternate-day injections, and 1 using a single administration. For mice, 21 methods were recorded, comprising 6 continuous, 13 alternate-day, and 2 single-injection protocols. Upon successful model induction, six behavioral pain alterations were consistently documented, evaluated using 16 different assessment techniques. Current PIPNP models predominantly utilize 6- to 8-week-old male Sprague-Dawley (SD) rats, with paclitaxel administered at 2 mg/kg on either days 0, 2, 4, and 6 or days 1, 3, 5, and 7. Based on data mining and comparative evaluation of modeling approaches, the use of 6- to 8-week-old male SD rats or 8- to 10-week-old male C57BL/6J mice with paclitaxel administered at 2 mg/kg on days 1, 3, 5, and 7 is recommended for establishing PIPNP models. The translational alignment between PIPNP models and clinical phenotypes remains insufficient and requires further refinement.
本研究旨在系统检索、综合和评估紫杉醇诱导的周围神经病理性疼痛（PIPNP）动物模型开发中所采用的方法，以优化模型构建和评估方案。在中国知网、万方和维普数据库中，使用检索词“Paclitaxel-induced peripheral neuropathic pain”或“Chemotherapy-induced peripheral neuropathic pain”以及“animal models”或“rats”或“mice”或“experimental animals”实施结构化检索策略。相同的检索标准应用于PubMed和Web of Science平台。文献检索涵盖从数据库建库至2024年12月31日的出版物。共审查了128篇文章，其中中文18篇，英文110篇。所有研究均采用啮齿动物模型，包括16个品系，涵盖雌雄两性，年龄范围为5周龄至10月龄。在大鼠中，确定了28种不同的建模方案：4种涉及连续注射紫杉醇，23种采用隔日注射，1种采用单次给药。对于小鼠，记录了21种方法，包括6种连续注射、13种隔日注射和2种单次注射方案。成功诱导模型后，一致记录到六种行为性疼痛改变，使用16种不同的评估技术进行评估。目前的PIPNP模型主要使用6至8周龄的雄性斯普拉格-道利（SD）大鼠，在第0、2、4和6天或第1、3、5和7天以2mg/kg的剂量给予紫杉醇。基于对建模方法的数据挖掘和比较评估，建议使用6至8周龄的雄性SD大鼠或8至10周龄的雄性C57BL/6J小鼠，在第1、3、5和7天以2mg/kg的剂量给予紫杉醇来建立PIPNP模型。PIPNP模型与临床表型之间的转化一致性仍然不足，需要进一步完善。
