Guo Xianting, Yu Yaqi, Xu Fan, Fu Kaiyun, Ding Xinhua, Guo Wenchao, Deng Jianyu, Rao Qiong
Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou, China.
Institute of Plant Protection, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences/ Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs/ Xinjiang Key Laboratory of Agricultural Biosafety, Urumqi, China.
Pest Manag Sci. 2025 Oct;81(10):6901-6910. doi: 10.1002/ps.70049. Epub 2025 Jul 16.
Carpomya pardalina, primarily found in certain regions of Eastern Europe, Central Asia and North Africa, is a highly serious pest that spreads rapidly. Its host plants have an extremely wide range, rendering C. pardalina highly vulnerable to long-distance spread through the import and export of agricultural products. Its invasion could have a devastating impact on relevant industries. Given the difficulty in differentiating Tephritidae pests based on morphological characteristics, a rapid and accurate molecular identification method is required to shorten the identification period of C. pardalina.
We have developed a loop-mediated isothermal amplification (LAMP) assay for C. pardalina. A set of LAMP primers was designed for the COX1 gene of C. pardalina. The LAMP amplification was capable of distinguishing C. pardalina from its similar species with excellent specificity, and the reaction demonstrated remarkable sensitivity, enabling the detection of a DNA template at a concentration of 0.969 ng/μL. Moreover, the amplification could be performed swiftly using the DNA extracted from crude DNA extractions as a template.
The detection can be completed within 75 min from DNA extraction, significantly enhancing the detection speed. Meanwhile, the visualization test demands simple equipment and is easy to operate, making it suitable for use at the port front line and under field working conditions. In this study, a set of rapid and accurate techniques was established based on the loop-mediated isothermal amplification (LAMP) technology for the detection of C. pardalina. © 2025 Society of Chemical Industry.
梨实蝇主要分布于东欧、中亚和北非的某些地区，是一种传播迅速的严重害虫。其寄主植物范围极广，这使得梨实蝇极易通过农产品进出口实现远距离传播。它的入侵可能会对相关产业造成毁灭性影响。鉴于基于形态特征区分实蝇科害虫存在困难，需要一种快速准确的分子鉴定方法来缩短梨实蝇的鉴定时间。
我们开发了一种针对梨实蝇的环介导等温扩增（LAMP）检测方法。针对梨实蝇的COX1基因设计了一组LAMP引物。LAMP扩增能够以优异的特异性将梨实蝇与其近缘物种区分开来，并且该反应表现出显著的灵敏度，能够检测浓度为0.969 ng/μL的DNA模板。此外，使用从粗提DNA提取物中提取的DNA作为模板可以快速进行扩增。
从DNA提取开始，检测可在75分钟内完成，显著提高了检测速度。同时，可视化检测所需设备简单且易于操作，适用于口岸一线和野外工作条件下使用。在本研究中，基于环介导等温扩增（LAMP）技术建立了一套快速准确的检测梨实蝇的技术方法。© 2025化学工业协会。
