Kou I L, Holmes R P
J Chromatogr. 1985 Aug 23;330(2):339-46. doi: 10.1016/s0021-9673(01)81991-9.
A method for the analysis of 25-hydroxycholesterol by high-performance liquid chromatography (HPLC) in a wide range of materials is described. Lipid extracts were initially purified on octadecyl silicic acid cartridges and by reversed-phase HPLC before quantitation by HPLC on a silicic acid column. The reproducibility of the method was confirmed by the analysis of plasma from rats fed 25-hydroxycholesterol. 25-Hydroxycholesterol was not detected in lard, cream, fresh egg yolk or spray-dried egg yolk powder. It was detected in egg yolk powder after heating at 110 degrees C for 4 days and its authenticity was confirmed by mass spectrometry.
本文描述了一种通过高效液相色谱法（HPLC）在多种材料中分析25-羟基胆固醇的方法。脂质提取物首先在十八烷基硅酸柱上进行纯化，然后通过反相HPLC进行纯化，最后在硅酸柱上通过HPLC进行定量分析。通过对喂食25-羟基胆固醇的大鼠血浆进行分析，证实了该方法的重现性。在猪油、奶油、新鲜蛋黄或喷雾干燥蛋黄粉中未检测到25-羟基胆固醇。在110℃加热4天后的蛋黄粉中检测到了25-羟基胆固醇，并通过质谱法确认了其真实性。
