用于定量一种新型抗心律失常药醋酸氟卡尼的间位-O-去烷基代谢物的高效液相色谱法。

High-performance liquid chromatographic method for the quantitation of a meta-O-dealkylated metabolite of flecainide acetate, a new antiarrhythmic.

作者信息

Chang S F, Welscher T M, Miller A M, McQuinn R L, Fox J M

出版信息

J Chromatogr. 1985 Sep 13;343(1):119-27. doi: 10.1016/s0378-4347(00)84574-0.

Abstract

A simple, sensitive and selective high-performance liquid chromatographic method has been developed to analyze meta-O-dealkylated flecainide, a major metabolite of flecainide acetate, in human biological fluids. Sample preparation is accomplished through the use of bonded-phase extraction columns and the samples are chromatographed on a reversed-phase system with fluorescence detection. An external calibration method is used for quantitation and the inter-day and intra-day precision and accuracy are good. The method has been used to determine metabolite levels in samples from healthy subjects and patients with arrhythmias or renal impairment.

摘要

已开发出一种简单、灵敏且具选择性的高效液相色谱法，用于分析醋酸氟卡尼的主要代谢物间位-O-去烷基氟卡尼在人体生物体液中的含量。通过使用键合相萃取柱来完成样品制备，样品在反相系统上进行色谱分离并采用荧光检测。采用外标法进行定量，日间和日内精密度及准确度良好。该方法已用于测定健康受试者以及患有心律失常或肾功能损害患者的样品中的代谢物水平。