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Belowground podcast: Digital droplet PCR to estimate root biomass and species profiling from soil samples.

作者信息

Khan Mamoona

机构信息

Assistant Features Editor, Plant Physiology, American Society of Plant Biologists.

Department of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn 53113, Germany.

出版信息

Plant Physiol. 2025 Jul 3;198(3). doi: 10.1093/plphys/kiaf313.

DOI:10.1093/plphys/kiaf313
PMID:40668939
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12301879/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d81f/12301879/5e876071736f/kiaf313f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d81f/12301879/5e876071736f/kiaf313f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d81f/12301879/5e876071736f/kiaf313f1.jpg

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本文引用的文献

1
Digital PCR enables direct root biomass quantification and species profiling in soil samples.数字PCR可实现土壤样本中根系生物量的直接定量和物种分析。
Plant Physiol. 2025 Jul 3;198(3). doi: 10.1093/plphys/kiaf276.
2
Digital PCR for the characterization of reference materials.用于参考物质表征的数字PCR
Mol Aspects Med. 2024 Apr;96:101256. doi: 10.1016/j.mam.2024.101256. Epub 2024 Feb 14.
3
Root traits as drivers of plant and ecosystem functioning: current understanding, pitfalls and future research needs.根系性状作为植物和生态系统功能的驱动因素：当前的认识、陷阱与未来研究需求
New Phytol. 2021 Nov;232(3):1123-1158. doi: 10.1111/nph.17072. Epub 2021 Jan 15.
4
Molecular thresholds of ITS2 and their implications for molecular evolution and species identification in seed plants.ITS2 的分子阈值及其对种子植物分子进化和物种鉴定的意义。
Sci Rep. 2017 Dec 11;7(1):17316. doi: 10.1038/s41598-017-17695-2.
5
Going underground: root traits as drivers of ecosystem processes.遁入地下：根系特性作为生态系统过程的驱动因素。
Trends Ecol Evol. 2014 Dec;29(12):692-9. doi: 10.1016/j.tree.2014.10.006. Epub 2014 Nov 11.
6
Considerations for digital PCR as an accurate molecular diagnostic tool.考虑将数字 PCR 作为一种准确的分子诊断工具。
Clin Chem. 2015 Jan;61(1):79-88. doi: 10.1373/clinchem.2014.221366. Epub 2014 Oct 22.
7
Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification.评估用于 DNA 拷贝数定量的液滴数字聚合酶链反应格式。
Anal Chem. 2012 Jan 17;84(2):1003-11. doi: 10.1021/ac202578x. Epub 2011 Dec 21.
8
High-throughput droplet digital PCR system for absolute quantitation of DNA copy number.高通量液滴数字 PCR 系统用于绝对定量 DNA 拷贝数。
Anal Chem. 2011 Nov 15;83(22):8604-10. doi: 10.1021/ac202028g. Epub 2011 Oct 28.
9
The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.MIQE指南：实时定量PCR实验发表的最低信息要求
Clin Chem. 2009 Apr;55(4):611-22. doi: 10.1373/clinchem.2008.112797. Epub 2009 Feb 26.
10
Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology.基于定量PCR（Q-PCR）的方法在微生物生态学中的优势与局限
FEMS Microbiol Ecol. 2009 Jan;67(1):6-20. doi: 10.1111/j.1574-6941.2008.00629.x.