Hisada Aya, Tanaka Yu-Ki, Eguchi Akifumi, Yamamoto Midori, Sakurai Kenichi, Todaka Emiko, Suzuki Noriyuki, Ogra Yasumitsu, Kasamatsu Atsushi, Uzawa Katsuhiro, Mori Chisato
Division of Environmental Preventive Medical Sciences, Center for Preventive Medical Sciences, Chiba University, Chiba, Japan.
Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan.
J Nutr Biochem. 2025 Nov;145:110035. doi: 10.1016/j.jnutbio.2025.110035. Epub 2025 Jul 14.
Deciduous teeth are a unique medium that record exposure to various elements over time, as they are formed during fetal and infantile periods. We aimed to evaluate the association between infant feeding methods and changes in the distribution of Barium (Ba) and Strontium (Sr) in deciduous teeth of children. The current analysis included 216 children around 6-7 years of age whose deciduous central incisor teeth were provided between March 2018 and February 2020, as an adjunct study of the Japan Environment and Children's study. Ba, Sr, and Calcium (Ca) levels in the deciduous teeth of these children were measured using laser ablation-inductively coupled plasma-mass spectrometry. We estimated the association between feeding methods or season of birth and the Ba to Ca (Ba:Ca) and Sr to Ca (Sr:Ca) ratios using multivariable linear mixed effects models. The Ba:Ca and Sr:Ca ratios were higher in the formula feeding group than in the breastfeeding group during the postnatal period (Ba:Ca: β=0.242 [95% CI: 0.149-0.335], Sr:Ca: β=0.223 [95% CI: 0.160-0.287] for formula group) and prenatal period (Ba:Ca: β=0.145 [95% CI: 0.062-0.227], Sr:Ca: β=0.152 [95% CI: 0.093-0.210] for formula group). Furthermore, the Sr:Ca ratio tended to be higher for summer-born children than for winter-born ones. The Ba:Ca and Sr:Ca ratios in deciduous teeth can serve as useful biomarkers for estimating feeding methods. Deciduous central incisors may serve as useful indicators of longitudinal elemental exposure and nutritional status from the prenatal to postnatal periods.
乳牙是一种独特的介质，随着时间的推移记录了对各种元素的暴露情况，因为它们是在胎儿期和婴儿期形成的。我们旨在评估婴儿喂养方式与儿童乳牙中钡（Ba）和锶（Sr）分布变化之间的关联。当前分析纳入了216名6-7岁左右的儿童，他们的乳牙中切牙于2018年3月至2020年2月期间提供，作为日本环境与儿童研究的一项附属研究。使用激光烧蚀-电感耦合等离子体质谱法测量了这些儿童乳牙中的Ba、Sr和钙（Ca）水平。我们使用多变量线性混合效应模型估计喂养方式或出生季节与Ba与Ca（Ba:Ca）和Sr与Ca（Sr:Ca）比值之间的关联。产后时期，配方奶喂养组的Ba:Ca和Sr:Ca比值高于母乳喂养组（配方奶组Ba:Ca：β=0.242 [95%CI：0.149-0.335]，Sr:Ca：β=0.223 [95%CI：0.160-0.287]），产前时期也是如此（配方奶组Ba:Ca：β=0.145 [95%CI：0.062-0.227]，Sr:Ca：β=0.152 [95%CI：0.093-0.210]）。此外，夏季出生的儿童的Sr:Ca比值往往高于冬季出生的儿童。乳牙中的Ba:Ca和Sr:Ca比值可作为估计喂养方式的有用生物标志物。乳牙中切牙可能是从产前到产后纵向元素暴露和营养状况的有用指标。
