Kong Lingtan, Wang Ling, Jing Shaozhen, Wang Jing, Leung Chung-Hang, Wang Wanhe
Institute of Medical Research, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi, 710072, China.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao, China.
Anal Chim Acta. 2025 Sep 22;1368:344327. doi: 10.1016/j.aca.2025.344327. Epub 2025 Jun 16.
The production of hypochlorous acid (HClO) is well recognized as a biomarker for liver injury, however, existing HClO bioimaging probes often lack specificity. Inspired by the specific recognition of oxime and hydrazide groups for HClO, we developed a novel N-alkyhydrazone moiety as a recognition unit for HClO. This moiety was integrated into an iridium(III) complex, creating a mitochondria-targeted luminescence probe for HClO that leverages the lipophilicity and cationic characteristics of iridium(III) complexes. The probe offers advantages including high specificity over other reactive oxygen species (ROS), rapid response of within 5 min, as well as robust stability. Furthermore, it effectively images both endogenous and endogenous mitochondrial HClO in liver injury-modeled cells and demonstrates excellent penetration capabilities in 3D cell spheres, highlighting its potential for diagnosing liver injury.
次氯酸（HClO）的产生被公认为肝损伤的生物标志物，然而，现有的HClO生物成像探针往往缺乏特异性。受肟基和酰肼基对HClO的特异性识别启发，我们开发了一种新型的N-烷基腙部分作为HClO的识别单元。该部分被整合到铱（III）配合物中，创建了一种针对线粒体的HClO发光探针，利用了铱（III）配合物的亲脂性和阳离子特性。该探针具有对其他活性氧（ROS）具有高特异性、5分钟内快速响应以及强大稳定性等优点。此外，它能有效成像肝损伤模型细胞中的内源性和内源性线粒体HClO，并在3D细胞球中表现出优异的穿透能力，突出了其在诊断肝损伤方面的潜力。
