Koder Ahmet, Bulut Erdoğan, Arslan Murat, Ersoy Onur
Faculty of Medicine, Department of Otorhinolaryngology, Trakya University, Edirne, 22030, Turkey.
Faculty of Health Sciences, Department of Audiology, Trakya University, Edirne, Turkey.
Eur J Med Res. 2025 Jul 16;30(1):638. doi: 10.1186/s40001-025-02889-6.
This study investigated the therapeutic efficacy of locally applied platelet-rich fibrin (PRF) on experimentally induced traumatic tympanic membrane perforations in rats.
The study comprised 24 young-adult Sprague Dawley rats (48 ears) randomly allocated into three groups of eight rats each: control group (no intervention), myringotomy group (standard perforation), and myringotomy with PRF application group. Otomicroscopic examinations were performed on days 7 and 14 to evaluate tympanic membrane healing and myringosclerosis development. Two rats from each experimental group were randomly selected for killing on day 7, with the remaining specimens collected on day 14. Histopathological analysis assessed acute and chronic inflammation, lamina propria edema and congestion, sclerosis, fibroblastic reaction, and squamous epithelial layer thickness.
The PRF-treated group demonstrated superior healing outcomes compared to the myringotomy-only group. By day 14, complete tympanic membrane closure was achieved in all eight ears of the PRF group versus six of eight ears in the myringotomy group. Electrophysiological measurements revealed significantly improved signal-to-noise ratios in the PRF group, approaching normal levels by day 14 (p < 0.05). Histological examination showed reduced inflammation and better-organized tissue architecture in PRF-treated specimens.
PRF application significantly enhanced tympanic membrane healing, accelerated closure time, and improved histological outcomes in traumatic perforations. These findings suggest PRF represents a promising therapeutic option for traumatic tympanic membrane perforation repair, warranting further clinical investigation.
本研究调查了局部应用富血小板纤维蛋白（PRF）对实验诱导的大鼠外伤性鼓膜穿孔的治疗效果。
该研究包括24只年轻成年斯普拉格-道利大鼠（48只耳朵），随机分为三组，每组8只大鼠：对照组（无干预）、鼓膜切开术组（标准穿孔）和鼓膜切开术加PRF应用组。在第7天和第14天进行耳显微镜检查，以评估鼓膜愈合和鼓膜硬化的发展情况。每组随机选择两只大鼠在第7天处死，其余标本在第14天收集。组织病理学分析评估急性和慢性炎症、固有层水肿和充血、硬化、成纤维细胞反应以及鳞状上皮层厚度。
与仅行鼓膜切开术的组相比，PRF治疗组显示出更好的愈合结果。到第14天，PRF组的所有8只耳朵均实现了鼓膜完全闭合，而鼓膜切开术组的8只耳朵中有6只实现了闭合。电生理测量显示PRF组的信噪比显著提高，到第14天接近正常水平（p < 0.05）。组织学检查显示，PRF治疗的标本炎症减轻，组织结构更有序。
应用PRF可显著促进外伤性穿孔的鼓膜愈合，加快闭合时间，并改善组织学结果。这些发现表明，PRF是外伤性鼓膜穿孔修复的一种有前景的治疗选择，值得进一步进行临床研究。
